Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

D.J. Scheffers; C. Robichon; T. den Blaauwen; G.J. Haan; G.M. Koningstein; D.W. van Bloois; J. Beckwith; S. Luirink

doi:10.1128/JB.00723-07

Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

D.J. Scheffers
, C. Robichon
, T. den Blaauwen
, G.J. Haan
, G.M. Koningstein
, D.W. van Bloois
, J. Beckwith
, S. Luirink

Research output: Contribution to Journal › Article › Academic

Abstract

The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Original language	English
Pages (from-to)	7273-7280
Journal	Journal of Bacteriology
Volume	189
Issue number	20
DOIs	https://doi.org/10.1128/JB.00723-07
Publication status	Published - 2007

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title = "Contribution of the FtsQ transmembrane segment to loclization to the cell division site.",

abstract = "The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright {\textcopyright} 2007, American Society for Microbiology. All Rights Reserved.",

author = "D.J. Scheffers and C. Robichon and \{den Blaauwen\}, T. and G.J. Haan and G.M. Koningstein and \{van Bloois\}, D.W. and J. Beckwith and S. Luirink",

year = "2007",

doi = "10.1128/JB.00723-07",

language = "English",

volume = "189",

pages = "7273--7280",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

AU - Scheffers, D.J.

AU - Robichon, C.

AU - den Blaauwen, T.

AU - Haan, G.J.

AU - Koningstein, G.M.

AU - van Bloois, D.W.

AU - Beckwith, J.

AU - Luirink, S.

PY - 2007

Y1 - 2007

N2 - The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

AB - The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

U2 - 10.1128/JB.00723-07

DO - 10.1128/JB.00723-07

M3 - Article

SN - 0021-9193

VL - 189

SP - 7273

EP - 7280

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 20

ER -

Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

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