Controlled Release of BMP-2 from a Heparin-Conjugated Strontium-Substituted Nanohydroxyapatite/Silk Fibroin Scaffold for Bone Regeneration

S. Yan; L. Feng; Q. Zhu; W. Yang; Y. Lan; D. Li; Y. Liu; W. Xue; R. Guo; G. Wu

doi:10.1021/acsbiomaterials.8b00459

Controlled Release of BMP-2 from a Heparin-Conjugated Strontium-Substituted Nanohydroxyapatite/Silk Fibroin Scaffold for Bone Regeneration

S. Yan
, L. Feng
, Q. Zhu
, W. Yang
, Y. Lan
, D. Li
, Y. Liu
, W. Xue
, R. Guo
, G. Wu

Reconstructive Oral Care

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The objective of this study was to develop heparin-conjugated strontium-substituted hydroxyapatite/silk fibroin (Sr-nHAp/SF-Hep) scaffold loaded bone morphogenetic proteins-2 (BMP-2) with sustained release to improve bone regeneration. The average pore diameters and porosity of Sr-nHAp/SF scaffolds were respectively approximately 150 μm and 90%. The mechanical properties and thermostability of the Sr-nHAp/SF scaffolds were significantly stronger than those of the SF scaffold. The weight of composite scaffolds is higher than that of the SF scaffold in simulated body fluids. The Sr-nHAp/SF scaffold exhibited excellent biological function of bone marrow mesenchymal stem cell (BMSC) proliferation and adhesion. The expression of related osteogenic genes, including osteocalcin, osteopontion, and alkaline phosphatase activity was elevated by Sr-nHAp/SF-Hep-BMP-2 scaffold, which promoted the differentiation of BMSCs into osteoblasts. In vivo results showed that Sr-nHAp/SF-Hep-BMP-2 scaffolds enhanced bone mineral density and improved new bone regeneration, which was accomplished through microcomputed tomography (micro-CT) and histological and histochemical staining analysis. These results demonstrated Sr-nHAp/SF-Hep-BMP-2 scaffolds with favorable biocompatibility and good mechanical properties have great potential to repair bone defects.

Original language	English
Pages (from-to)	3291-3303
Journal	ACS Biomaterials Science & Engineering
Volume	4
Issue number	9
DOIs	https://doi.org/10.1021/acsbiomaterials.8b00459
Publication status	Published - 2018

Bibliographical note

With supplementary material.

Funding

This study was supported financially by the Natural Science Foundation of China (Grants 81573708 and 31271019), the Science Foundation of Guangdong Province (Grant 2015A030306049), the Science and Technology Program of Guangzhou (Grants 201601010270, 2017010160489, and 201704030083), the Pearl River S&T Nova Program of Guangzhou (Grants 201710010155 and 201806010072), Guangdong Special Support Program for High-level Talents (Grant 2015TQ01R588), the Discipline of Integrated Chinese and Western Medicine in Guangzhou University of Chinese Medicine (Grant YN2015MS21), and the Science and Technology Project of Guangdong province (Grants 2015A010101313, 2017A050506011, 2017A050501013, and 2017B090911012). This study was supported financially by the Natural Science Foundation of China (Grants 81573708 and 31271019), the Science Foundation of Guangdong Province (Grant 2015A030306049), the Science and Technology Program of Guangzhou (Grants 201601010270, 2017010160489, and 201704030083) the Pearl River S&T Nova Program of Guangzhou (Grants 201710010155 and 201806010072), Guangdong Special Support Program for High-level Talents (Grant 2015TQ01R588), the Discipline of Integrated Chinese and Western Medicine in Guangzhou University of Chinese Medicine (Grant YN2015MS21), and the Science and Technology Project of Guangdong province (Grants 2015A010101313, 2017A050506011, 2017A050501013, and 2017B090911012).

Funders	Funder number
Discipline of Integrated Chinese and Western Medicine in Guangzhou University of Chinese Medicine
Guangdong Special Support Program for High-level Talents
National Natural Science Foundation of China	81573708, 31271019
Natural Science Foundation of Guangdong Province	2015A030306049
Guangzhou Science and Technology Program key projects	201704030083, 201601010270, 2017010160489
Pearl River S and T Nova Program of Guangzhou	201806010072, 201710010155
Guangzhou University of Chinese Medicine	YN2015MS21
Science and Technology Planning Project of Guangdong Province	2017B090911012, 2015A010101313, 2017A050506011, 2017A050501013
Tip-top Scientific and Technical Innovative Youth Talents of Guangdong Special Support Program	2015TQ01R588

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@article{736885f14ee64f80846a3c77944a5bf2,

title = "Controlled Release of BMP-2 from a Heparin-Conjugated Strontium-Substituted Nanohydroxyapatite/Silk Fibroin Scaffold for Bone Regeneration",

abstract = "The objective of this study was to develop heparin-conjugated strontium-substituted hydroxyapatite/silk fibroin (Sr-nHAp/SF-Hep) scaffold loaded bone morphogenetic proteins-2 (BMP-2) with sustained release to improve bone regeneration. The average pore diameters and porosity of Sr-nHAp/SF scaffolds were respectively approximately 150 μm and 90\%. The mechanical properties and thermostability of the Sr-nHAp/SF scaffolds were significantly stronger than those of the SF scaffold. The weight of composite scaffolds is higher than that of the SF scaffold in simulated body fluids. The Sr-nHAp/SF scaffold exhibited excellent biological function of bone marrow mesenchymal stem cell (BMSC) proliferation and adhesion. The expression of related osteogenic genes, including osteocalcin, osteopontion, and alkaline phosphatase activity was elevated by Sr-nHAp/SF-Hep-BMP-2 scaffold, which promoted the differentiation of BMSCs into osteoblasts. In vivo results showed that Sr-nHAp/SF-Hep-BMP-2 scaffolds enhanced bone mineral density and improved new bone regeneration, which was accomplished through microcomputed tomography (micro-CT) and histological and histochemical staining analysis. These results demonstrated Sr-nHAp/SF-Hep-BMP-2 scaffolds with favorable biocompatibility and good mechanical properties have great potential to repair bone defects.",

author = "S. Yan and L. Feng and Q. Zhu and W. Yang and Y. Lan and D. Li and Y. Liu and W. Xue and R. Guo and G. Wu",

note = "With supplementary material.",

year = "2018",

doi = "10.1021/acsbiomaterials.8b00459",

language = "English",

volume = "4",

pages = "3291--3303",

journal = "ACS Biomaterials Science \& Engineering",

issn = "2373-9878",

publisher = "American Chemical Society",

number = "9",

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TY - JOUR

T1 - Controlled Release of BMP-2 from a Heparin-Conjugated Strontium-Substituted Nanohydroxyapatite/Silk Fibroin Scaffold for Bone Regeneration

AU - Yan, S.

AU - Feng, L.

AU - Zhu, Q.

AU - Yang, W.

AU - Lan, Y.

AU - Li, D.

AU - Liu, Y.

AU - Xue, W.

AU - Guo, R.

AU - Wu, G.

N1 - With supplementary material.

PY - 2018

Y1 - 2018

N2 - The objective of this study was to develop heparin-conjugated strontium-substituted hydroxyapatite/silk fibroin (Sr-nHAp/SF-Hep) scaffold loaded bone morphogenetic proteins-2 (BMP-2) with sustained release to improve bone regeneration. The average pore diameters and porosity of Sr-nHAp/SF scaffolds were respectively approximately 150 μm and 90%. The mechanical properties and thermostability of the Sr-nHAp/SF scaffolds were significantly stronger than those of the SF scaffold. The weight of composite scaffolds is higher than that of the SF scaffold in simulated body fluids. The Sr-nHAp/SF scaffold exhibited excellent biological function of bone marrow mesenchymal stem cell (BMSC) proliferation and adhesion. The expression of related osteogenic genes, including osteocalcin, osteopontion, and alkaline phosphatase activity was elevated by Sr-nHAp/SF-Hep-BMP-2 scaffold, which promoted the differentiation of BMSCs into osteoblasts. In vivo results showed that Sr-nHAp/SF-Hep-BMP-2 scaffolds enhanced bone mineral density and improved new bone regeneration, which was accomplished through microcomputed tomography (micro-CT) and histological and histochemical staining analysis. These results demonstrated Sr-nHAp/SF-Hep-BMP-2 scaffolds with favorable biocompatibility and good mechanical properties have great potential to repair bone defects.

AB - The objective of this study was to develop heparin-conjugated strontium-substituted hydroxyapatite/silk fibroin (Sr-nHAp/SF-Hep) scaffold loaded bone morphogenetic proteins-2 (BMP-2) with sustained release to improve bone regeneration. The average pore diameters and porosity of Sr-nHAp/SF scaffolds were respectively approximately 150 μm and 90%. The mechanical properties and thermostability of the Sr-nHAp/SF scaffolds were significantly stronger than those of the SF scaffold. The weight of composite scaffolds is higher than that of the SF scaffold in simulated body fluids. The Sr-nHAp/SF scaffold exhibited excellent biological function of bone marrow mesenchymal stem cell (BMSC) proliferation and adhesion. The expression of related osteogenic genes, including osteocalcin, osteopontion, and alkaline phosphatase activity was elevated by Sr-nHAp/SF-Hep-BMP-2 scaffold, which promoted the differentiation of BMSCs into osteoblasts. In vivo results showed that Sr-nHAp/SF-Hep-BMP-2 scaffolds enhanced bone mineral density and improved new bone regeneration, which was accomplished through microcomputed tomography (micro-CT) and histological and histochemical staining analysis. These results demonstrated Sr-nHAp/SF-Hep-BMP-2 scaffolds with favorable biocompatibility and good mechanical properties have great potential to repair bone defects.

U2 - 10.1021/acsbiomaterials.8b00459

DO - 10.1021/acsbiomaterials.8b00459

M3 - Article

SN - 2373-9878

VL - 4

SP - 3291

EP - 3303

JO - ACS Biomaterials Science & Engineering

JF - ACS Biomaterials Science & Engineering

IS - 9

ER -

Controlled Release of BMP-2 from a Heparin-Conjugated Strontium-Substituted Nanohydroxyapatite/Silk Fibroin Scaffold for Bone Regeneration

Abstract

Bibliographical note

Funding

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