Coordinated behavior of mitochondria in both space and time: a reactive species-activated wave of mitochondrial depolarization.

N. Brady, S.P. Elmore, J.H.G.M. van Beek, K. Krab, P.J. Courtoy, L. Hue, H.V. Westerhoff

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Abstract

Reactive oxygen species (ROS) can trigger a transient burst of mitochondrial ROS production via ROS activation of the mitochondrial permeability transition pore (MPTP), a phenomenon termed ROS-induced ROS release (RIRR). The goal of this study was to investigate if the generation of ROS in a discrete region of a cardiomyocyte could serve to propagate RIRR-mediated mitochondrial depolarizations throughout a cell. Our experiments revealed that localized RIRR activated either RIRR-mediated fluctuations in mitochondrial membrane potential (time period: 3-10 min) or a traveling wave of depolarization of the cell's mitochondria (velocity: ∼5 μm/min). Both phenomena appeared to be mediated by the mitochondrial permeability transition pore and eventually encompassed the majority of the mitochondrial population of both isolated rat and rabbit cardiomyocytes. Furthermore, depolarization was often reversible; the waves of depolarization were then followed by a rapid (∼40 μm/min) repolarization wave of the mitochondria. We show that the RIRR can function to communicate the mitochondrial permeability transition from one mitochondrion to another in the isolated adult cardiomyocyte.
Original languageEnglish
Pages (from-to)2022-2034
Number of pages13
JournalBiophysical Journal
Volume87
DOIs
Publication statusPublished - 2004

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