Coproporphyrin excretion by Azorhizobium caulinodans under micro-aerobic conditions.

A.F. Pronk, J. Stigter, A.H. Stouthamer, F.J. de Bruijn, F.C. Boogerd

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Azorhizobium caulinodans ORS571 was found to excrete moderate amounts of a fluorescent pigment into the culture medium in response to dissolved oxygen tensions below 1.0 kPa. The pigment was identified as coproporphyrin, on the basis of its optical and fluorescence spectra. FixLJ and fixK mutant derivatives of ORS571 were found to excrete 25-fold higher amounts of coproporphyrin under micro-aerobic conditions than the wild type strain. These observations suggest that A. caulinodans switches from an aerobic to an anaerobic coproporphyrinogen oxidase when the dissolved oxygen tension falls below 1.0 kPa and that the fixLJ and fixK genes are involved in the regulation of expression of the anaerobic coproporphyrinogen oxidase.
Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalAntonie van Leeuwenhoek
Volume74
DOIs
Publication statusPublished - 1998

Fingerprint Dive into the research topics of 'Coproporphyrin excretion by Azorhizobium caulinodans under micro-aerobic conditions.'. Together they form a unique fingerprint.

Cite this