Coralline hydroxyapatite coated with a layer biomimetic calcium phosphate containing bmp-2 induces dose-related ectopic bone formation in wistar rats

Henri J.J. Uijlenbroek, Xingnan Lin, Xin Zhang, Liquan Deng, Daniel Wismeijer, Mingjie Wang, Lingfei Wei, Yuanna Zheng, Yuelian Liu*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In order to evaluate loading methods and the dose dependency of bone morphogenetic protein 2 (BMP-2) in ectopic bone formation, an osteoinductive material consisting of commercially available coralline hydroxyapatite (CHA) was coated with a layer of biomimetic calcium phosphate (BioCaP) containing BMP-2 in different ways. Eight groups—each containing samples of 0.25 g CHA—were formed and coated with, respectively, BioCaP with internally incorporated BMP-2 in concentrations of 1, 5, 10, 20, 40 and 60 µg per sample, and the two control groups with BioCaP only and BioCaP with 20 µg of adsorbed BMP-2 per sample. The samples were implanted subcutaneously in 27 male Wistar rats. The histological results show that there is no bone formation in the group in which no BMP-2 was included. All samples with BioCaP containing BMP-2 show bone formation. The group with 20 µg of adsorbed BMP-2 per sample shows the least bone formation. Coating-incorporated BMP-2 is more efficient in inducing bone formation than adsorbed BMP-2. The group with 5 µg of coating-incorporated BMP-2 per sample shows the most bone formation. Increasing the amount of coating-incorporated BMP-2 up to 60 µg does not improve ectopic bone formation.

Original languageEnglish
Article number1195
Pages (from-to)1-13
Number of pages13
JournalCoatings
Volume11
Issue number10
Early online date30 Sep 2021
DOIs
Publication statusPublished - Oct 2021

Bibliographical note

Funding Information:
Funding: This research was funded by Uijlenbroek & Partners, dentists. This project was practically supported by the Zhejiang Provincial Natural Science Foundation of China (LQ19H280008) and Shandong Taishan Scholar Program to Y.L.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • BioCaP
  • Biomimetic calcium phosphate
  • BMP-2
  • Bone morphogenetic protein-2
  • CHA
  • Coralline hydroxyapatite
  • Ectopic bone formation
  • Osteoinduction

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