Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17

Katsiaryna Tratsiak; Oksana Degtjarik; Ivana Drienovska; Lukas Chrast; Pavlina Rezacova; Michal Kuty; Radka Chaloupkova; Jiri Damborsky; Ivana Kuta Smatanova

doi:10.1107/S1744309113012979

Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17

Katsiaryna Tratsiak, Oksana Degtjarik, Ivana Drienovska, Lukas Chrast, Pavlina Rezacova, Michal Kuty, Radka Chaloupkova, Jiri Damborsky, Ivana Kuta Smatanova^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 Å.

Original language	English
Pages (from-to)	683-688
Number of pages	6
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	69
Issue number	6
DOIs	https://doi.org/10.1107/S1744309113012979
Publication status	Published - 1 Jan 2013
Externally published	Yes

Keywords

DmxA
DpcA
haloalkane dehalogenases
Marinobacter sp. ELB17
Psychrobacter cryohalolentis K5

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Tratsiak, K., Degtjarik, O., Drienovska, I., Chrast, L., Rezacova, P., Kuty, M., Chaloupkova, R., Damborsky, J., & Kuta Smatanova, I. (2013). Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69(6), 683-688. https://doi.org/10.1107/S1744309113012979

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title = "Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17",

abstract = "Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 {\AA}.",

keywords = "DmxA, DpcA, haloalkane dehalogenases, Marinobacter sp. ELB17, Psychrobacter cryohalolentis K5",

author = "Katsiaryna Tratsiak and Oksana Degtjarik and Ivana Drienovska and Lukas Chrast and Pavlina Rezacova and Michal Kuty and Radka Chaloupkova and Jiri Damborsky and {Kuta Smatanova}, Ivana",

year = "2013",

month = jan,

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doi = "10.1107/S1744309113012979",

language = "English",

volume = "69",

pages = "683--688",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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Tratsiak, K, Degtjarik, O, Drienovska, I, Chrast, L, Rezacova, P, Kuty, M, Chaloupkova, R, Damborsky, J & Kuta Smatanova, I 2013, 'Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 69, no. 6, pp. 683-688. https://doi.org/10.1107/S1744309113012979

Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17. / Tratsiak, Katsiaryna; Degtjarik, Oksana; Drienovska, Ivana et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 6, 01.01.2013, p. 683-688.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Crystallographic analysis of new psychrophilic haloalkane dehalogenases

T2 - DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17

AU - Tratsiak, Katsiaryna

AU - Degtjarik, Oksana

AU - Drienovska, Ivana

AU - Chrast, Lukas

AU - Rezacova, Pavlina

AU - Kuty, Michal

AU - Chaloupkova, Radka

AU - Damborsky, Jiri

AU - Kuta Smatanova, Ivana

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 Å.

AB - Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 Å.

KW - DmxA

KW - DpcA

KW - haloalkane dehalogenases

KW - Marinobacter sp. ELB17

KW - Psychrobacter cryohalolentis K5

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VL - 69

SP - 683

EP - 688

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 6

ER -

Tratsiak K, Degtjarik O, Drienovska I, Chrast L, Rezacova P, Kuty M et al. Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17. Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 Jan 1;69(6):683-688. doi: 10.1107/S1744309113012979

Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17

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Keywords

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