Cutting edge: GPR35/CXCR8 is the receptor of the mucosal chemokine CXCL17

José L Maravillas-Montero, Amanda M Burkhardt, Peter A Hevezi, Christina D Carnevale, Martine J Smit, Albert Zlotnik

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Chemokines are chemotactic cytokines that direct the traffic of leukocytes and other cells in the body. Chemokines bind to G protein-coupled receptors expressed on target cells to initiate signaling cascades and induce chemotaxis. Although the cognate receptors of most chemokines have been identified, the receptor for the mucosal chemokine CXCL17 is undefined. In this article, we show that GPR35 is the receptor of CXCL17. GPR35 is expressed in mucosal tissues, in CXCL17-responsive monocytes, and in the THP-1 monocytoid cell line. Transfection of GPR35 into Ba/F3 cells rendered them responsive to CXCL17, as measured by calcium-mobilization assays. Furthermore, GPR35 expression is downregulated in the lungs of Cxcl17(-/-) mice, which exhibit defects in macrophage recruitment to the lungs. We conclude that GPR35 is a novel chemokine receptor and suggest that it should be named CXCR8.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalJournal of Immunology
Volume194
Issue number1
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Amino Acid Sequence
  • Animals
  • Cell Line, Tumor
  • Chemokines
  • Chemokines, CXC
  • Chemotaxis, Leukocyte
  • Humans
  • Lung
  • Macrophages
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • Monocytes
  • Mucous Membrane
  • Protein Structure, Tertiary
  • Receptors, G-Protein-Coupled
  • Sequence Alignment
  • Transfection
  • Journal Article
  • Research Support, N.I.H., Extramural

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