D-LL-31 in combination with ceftazidime synergistically enhances bactericidal activity and biofilm destruction in Burkholderia pseudomallei

S. Wongkaewkhiaw, S. Taweechaisupapong, C. Anutrakunchai, K. Nazmi, J.G.M. Bolscher, S. Wongratanacheewin, S. Kanthawong

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Melioidosis is a severe disease caused by Burkholderia pseudomallei. The biofilm of B. pseudomallei acquires resistance to several antibiotics and may be related to relapse in melioidosis patients. Here, the killing activity of antimicrobial peptides (LL-37, LL-31) and the D-enantiomers (D-LL-37, D-LL-31) in combination with ceftazidime (CAZ) against B. pseudomallei 1026b, H777 and a biofilm mutant M10, derived from H777 grown under biofilm-stimulating conditions was observed. Using static conditions, D-LL-31 exhibited the strongest killing activity against the three isolates in a dose-dependent manner. IC50 values for D-LL-31 ranged from 1 to 6 µM, for isolates M10, H777, and 1026b, respectively. Moreover, D-LL-31 combined with CAZ synergistically decreased the IC50 values of the peptide and antibiotic and caused also disruption of biofilms of B. pseudomallei 1026b under flow conditions. Thus a combination of D-LL-31 and CAZ may enhance the efficacy of the currently used antibiotic treatments against B. pseudomallei.
Original languageEnglish
Pages (from-to)573-584
JournalBIOFOULING
Volume35
Issue number5
DOIs
Publication statusPublished - 2019

Bibliographical note

Export Date: 22 October 2019

CODEN: BFOUE

Funding

This study was supported by a grant fund under the Thailand Research Fund (TRF), Bangkok [grant number MRG5980045] and the Melioidosis Research Center (MRC), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. CA is supported by a grant from the Post-Doctoral Training Program from Research Affairs and Graduate School, Khon Kaen University, Thailand [grant number 58437]. The authors would like to acknowledge Prof. David Blair for editing the MS via Publication Clinic KKU, Thailand.

FundersFunder number
Melioidosis Research Center
Khon Kaen University58437
Thailand Research FundMRG5980045

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