@article{4ef83643688f45b9a3387f45a54dfad1,
title = "Decoding the protein composition of whole nucleosomes with Nuc-MS",
abstract = "Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts of the {\textquoteleft}histone code{\textquoteright}. To preserve nucleosome-level information, we developed Nuc-MS, which displays the landscape of histone variants and their post-translational modifications (PTMs) in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (sixfold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (for example, a >15-fold enrichment of dimethylated H3K79me2). Nuc-MS is highly concordant with chromatin immunoprecipitation-sequencing (ChIP-seq) and offers a new readout of nucleosome-level biology.",
author = "Schachner, {Luis F.} and Kevin Joo{\ss} and Morgan, {Marc A.} and Andrea Piunti and Meiners, {Matthew J.} and Kafader, {Jared O.} and Lee, {Alexander S.} and Marta Iwanaszko and Cheek, {Marcus A.} and Burg, {Jonathan M.} and Howard, {Sarah A.} and Michael-Christopher Keogh and Ali Shilatifard and Kelleher, {Neil L.}",
year = "2021",
month = mar,
day = "1",
doi = "10.1038/s41592-020-01052-9",
language = "English",
volume = "18",
pages = "303--308",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "3",
}
