TY - JOUR
T1 - Decreased mineralization and increased calcium release in isolated fetal mouse long bones under near weightlessness
AU - Van Loon, Jack J.W.A.
AU - Bervoets, Dirk‐Jan ‐J
AU - Burger, Elisabeth H.
AU - Dieudonn É, Suzanne C.
AU - Hagen, Jan‐Willem ‐W
AU - Semeins, Cor M.
AU - Doulabi, Behrouz Zandieh
AU - Veldhuijzen, Paul J.
PY - 1995/4
Y1 - 1995/4
N2 - Mechanical loading plays an important role in the development and maintenance of skeletal tissues. Subnormal mechanical stress as a result of bed rest, immobilization, but also in spaceflight, results in a decreased bone mass and disuse osteoporosis, whereas supranormal loads upon extremities result in an increased bone mass. In this first in vitro experiment with complete fetal mouse cartilaginous long bones, cultured under microgravity conditions, we studied growth, glucose utilization, collagen synthesis, and mineral metabolism, during a 4‐day culture period in space. There was no change in percent length increase and collagen synthesis under microgravity compared with in‐flight 1× gravity. Glucose utilization and mineralization were decreased under microgravity. In addition, mineral resorption, as measured by 45Ca release, was increased. These data suggest that weightlessness has modulating effects on skeletal tissue cells. Loss of bone during spaceflight could be the result of both impaired mineralization as well as increased resorption.
AB - Mechanical loading plays an important role in the development and maintenance of skeletal tissues. Subnormal mechanical stress as a result of bed rest, immobilization, but also in spaceflight, results in a decreased bone mass and disuse osteoporosis, whereas supranormal loads upon extremities result in an increased bone mass. In this first in vitro experiment with complete fetal mouse cartilaginous long bones, cultured under microgravity conditions, we studied growth, glucose utilization, collagen synthesis, and mineral metabolism, during a 4‐day culture period in space. There was no change in percent length increase and collagen synthesis under microgravity compared with in‐flight 1× gravity. Glucose utilization and mineralization were decreased under microgravity. In addition, mineral resorption, as measured by 45Ca release, was increased. These data suggest that weightlessness has modulating effects on skeletal tissue cells. Loss of bone during spaceflight could be the result of both impaired mineralization as well as increased resorption.
UR - http://www.scopus.com/inward/record.url?scp=0028954087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028954087&partnerID=8YFLogxK
U2 - 10.1002/jbmr.5650100407
DO - 10.1002/jbmr.5650100407
M3 - Article
C2 - 7610925
AN - SCOPUS:0028954087
SN - 0884-0431
VL - 10
SP - 550
EP - 557
JO - Journal of Bone and Mineral Research
JF - Journal of Bone and Mineral Research
IS - 4
ER -