Dentistry and OMICS: Transcriptome dynamics of an oral ecosystem as measured by changes in oral polymorphonuclear neutrophils in experimental gingivitis

P. Rijkschroeff, T. Schoenmaker, M. Caspers, L. Verschuren, B.J.F. Keijser, E.A. Nicu, B.G. Loos

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Oral health and dentistry are essential components of systems medicine, which has received lesser attention in comparison to other medical fields, such as cancer biology. In this context, oral polymorphonuclear neutrophils (oPMNs) play an important role in the maintenance of oral health. To the best of our knowledge, this is the first study to report original observations on the transcriptional responses of oPMNs during experimentally induced gingivitis, by temporarily refraining from regular oral care. Oral rinses were prospectively collected at four different time points for oPMNs isolation from healthy volunteers: day 1 (start of the experimental gingivitis challenge), day 9 (during challenge), day 14 (end of the challenge), and day 21 (postchallenge). Transcriptome of oPMNs was determined by RNA sequencing. Differentially expressed genes (DEGs) were selected at p < 0.01 level, and evaluated for pathway regulation using Ingenuity Pathway Analysis suite. We found four major clusters of DEGs, consisting of 256 initial response DEGs (day 9 only), 221 late response DEGs (day 14 only), 53 persistent responsive DEGs (consistent at day 9 and 14), and 524 DEGs showing responses only in the postchallenge phase (day 21 only). Pathway analysis of the initial and late response DEGs showed involvement in many immune regulatory pathways and PMN function, whereas DEGs at day 21 were associated with epithelial adherence signaling and other miscellaneous related signaling pathways. The results from this pilot study showed that oPMNs mediate oral inflammatory processes, suggesting their immunomodulatory role in oral equilibrium.

Original languageEnglish
Pages (from-to)531-540
Number of pages10
JournalOMICS: A Journal of Integrative Biology
Volume24
Issue number9
DOIs
Publication statusPublished - Sept 2020

Funding

The Departments of Periodontology and Preventive Dentistry have been supported by a grant from the University of Amsterdam for research into the focal point ‘‘Oral Infections and Inflammation.’’ The study was financed in part by the Academic Center for Dentistry Amsterdam Dental Research BV (ADR). Academic Center for Dentistry Amsterdam Dental Research BV has received funding from TI Food and Nutrition, a public/private partnership on precompetitive research in food and nutrition. Funding of this original research did not affect nor did it play a role in the conception of the study, the design, data acquisition, data analyses, and interpretation of the data.

FundersFunder number
Academic Center for Dentistry Amsterdam Dental Research BV
TI Food and Nutrition
Universiteit van Amsterdam

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