Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: Comparison of derivatization with Ellman's reagent and monobromobimane.

F.E.C. Sneller, L.M. van Heerwaarden, P.L.M. Koevoets

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Phytochelatins (PCs) are a family of thiol-rich peptides, with the general structure (γ-Glu-Cys)(n)-Gly, with n = 2-11, induced in plants upon exposure to excessive amounts of heavy metals and some metalloids, such as arsenic. Two types of PC analyses are currently used, i.e., acid extraction and separation on HPLC with either precolumn derivatization (pH 8.2) with monobromobimane (mBBr) or postcolumn derivatization (pH 7.8) with Ellman's reagent [5,5'-dithiobis(2-nitrobenzoic acid), DTNB]. Although both methods were satisfactory for analysis of Cd-induced PCs, formation of (RS)
Original languageEnglish
Pages (from-to)4014-4019
JournalJournal of Agricultural and Food Chemistry
Volume48
Issue number9
DOIs
Publication statusPublished - 2000

Fingerprint

Dive into the research topics of 'Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: Comparison of derivatization with Ellman's reagent and monobromobimane.'. Together they form a unique fingerprint.

Cite this