Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction

D.O. Visscher; A. Gleadall; J.K. Buskermolen; F. Burla; J. Segal; G.H. Koenderink; M.N. Helder; P.P.M. van Zuijlen

doi:10.1002/jbm.b.34264

Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction

D.O. Visscher, A. Gleadall, J.K. Buskermolen, F. Burla, J. Segal, G.H. Koenderink, M.N. Helder, P.P.M. van Zuijlen

Maxillofacial Surgery (VUmc)

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D-printed poly-ε-caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neocartilage formation, and printing accuracy), a hybrid auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced cartilage-like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p >.05). The final auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid auricular mold appears to be an adequate model for cartilage tissue engineering and may provide a novel approach to auricular cartilage regeneration for facial reconstruction.

Original language	English
Pages (from-to)	1711-1721
Number of pages	11
Journal	Journal of Biomedical Materials Research Part B Applied Biomaterials
Volume	107
Issue number	5
DOIs	https://doi.org/10.1002/jbm.b.34264
Publication status	Published - 1 Jul 2019

Funding

This work was financially supported by the Dutch Burn Foundation (project number 15.107). The authors would like to thank Mr. Sjoerd te Slaa and Mr. Niels Liberton for help with computer aided design. In addition, the authors acknowledge Nanne Paauw, Esther Hendrickx, and Sander Spiekstra for help with imaging of the alginate beads. The authors declare that they have no competing interests.

Funders	Funder number
Nederlandse Brandwonden Stichting	15.107

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10.1002/jbm.b.34264

https://hdl.handle.net/11245.1/ee3cac2b-418e-4936-80b8-db3e4a97aa9a

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Visscher, D. O., Gleadall, A., Buskermolen, J. K., Burla, F., Segal, J., Koenderink, G. H., Helder, M. N., & van Zuijlen, P. P. M. (2019). Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction. Journal of Biomedical Materials Research Part B Applied Biomaterials, 107(5), 1711-1721. https://doi.org/10.1002/jbm.b.34264

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title = "Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction",

abstract = "The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D-printed poly-ε-caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neocartilage formation, and printing accuracy), a hybrid auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced cartilage-like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p >.05). The final auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid auricular mold appears to be an adequate model for cartilage tissue engineering and may provide a novel approach to auricular cartilage regeneration for facial reconstruction.",

author = "D.O. Visscher and A. Gleadall and J.K. Buskermolen and F. Burla and J. Segal and G.H. Koenderink and M.N. Helder and {van Zuijlen}, P.P.M.",

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Visscher, DO, Gleadall, A, Buskermolen, JK, Burla, F, Segal, J, Koenderink, GH, Helder, MN & van Zuijlen, PPM 2019, 'Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction', Journal of Biomedical Materials Research Part B Applied Biomaterials, vol. 107, no. 5, pp. 1711-1721. https://doi.org/10.1002/jbm.b.34264

Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction. / Visscher, D.O.; Gleadall, A.; Buskermolen, J.K. et al.
In: Journal of Biomedical Materials Research Part B Applied Biomaterials, Vol. 107, No. 5, 01.07.2019, p. 1711-1721.

Research output: Contribution to Journal › Article › Academic › peer-review

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T1 - Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction

AU - Visscher, D.O.

AU - Gleadall, A.

AU - Buskermolen, J.K.

AU - Burla, F.

AU - Segal, J.

AU - Koenderink, G.H.

AU - Helder, M.N.

AU - van Zuijlen, P.P.M.

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N2 - The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D-printed poly-ε-caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neocartilage formation, and printing accuracy), a hybrid auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced cartilage-like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p >.05). The final auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid auricular mold appears to be an adequate model for cartilage tissue engineering and may provide a novel approach to auricular cartilage regeneration for facial reconstruction.

AB - The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D-printed poly-ε-caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neocartilage formation, and printing accuracy), a hybrid auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced cartilage-like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p >.05). The final auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid auricular mold appears to be an adequate model for cartilage tissue engineering and may provide a novel approach to auricular cartilage regeneration for facial reconstruction.

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M3 - Article

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SP - 1711

EP - 1721

JO - Journal of Biomedical Materials Research Part B Applied Biomaterials

JF - Journal of Biomedical Materials Research Part B Applied Biomaterials

IS - 5

ER -

Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for auricular cartilage reconstruction

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