Design of Artificial Enzymes: Insights into Protein Scaffolds

Stefanie Hanreich, Elisa Bonandi, Ivana Drienovská*

*Corresponding author for this work

Research output: Contribution to JournalReview articleAcademicpeer-review

Abstract

The design of artificial enzymes has emerged as a promising tool for the generation of potent biocatalysts able to promote new-to-nature reactions with improved catalytic performances, providing a powerful platform for wide-ranging applications and a better understanding of protein functions and structures. The selection of an appropriate protein scaffold plays a key role in the design process. This review aims to give a general overview of the most common protein scaffolds that can be exploited for the generation of artificial enzymes. Several examples are discussed and categorized according to the strategy used for the design of the artificial biocatalyst, namely the functionalization of natural enzymes, the creation of a new catalytic site in a protein scaffold bearing a wide hydrophobic pocket and de novo protein design. The review is concluded by a comparison of these different methods and by our perspective on the topic.

Original languageEnglish
Article numbere202200566
Pages (from-to)1-18
Number of pages18
JournalChemBioChem
Volume24
Issue number6
Early online date23 Nov 2022
DOIs
Publication statusPublished - 14 Mar 2023

Bibliographical note

Funding Information:
Authors acknowledge financial support from the Netherlands Organisation for Scientific Research (NWO VI.Veni.202.166).

Publisher Copyright:
© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.

Keywords

  • artificial enzymes
  • enzyme engineering
  • metalloenzymes
  • protein design
  • protein scaffolds

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