Detection of flowing fluorescent particles in a microcapillary using fluorescence correlation spectroscopy.

B.H. Kunst, A. Schots, A.J.W.G. Visser

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Capillary flow experiments are described with fluorescent molecules, bacteria, and microspheres using fluorescence correlation spectroscopy as an analytical tool. The flow velocity in the microcapillary is determined by fitting autocorrelation traces with a model containing parameters related to diffusion and flow. The flow profile of pressure-driven flow inside a microcapillary is determined by using the fluorescence fluctuations of a small dye molecule. It was found that bacteria and microspheres are retarded in their flow by optical forces produced by the laser beam.
    Original languageEnglish
    Pages (from-to)5350-5357
    JournalAnalytical Chemistry
    Volume74
    DOIs
    Publication statusPublished - 2002

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