Abstract
The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.
Original language | English |
---|---|
Pages (from-to) | 109-19 |
Number of pages | 11 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1095 |
DOIs | |
Publication status | Published - 2014 |
Externally published | Yes |
Keywords
- DNA Replication
- DNA, Single-Stranded/metabolism
- MicroRNAs/genetics
- Nucleic Acid Amplification Techniques/methods
- Phosphorylation
- RNA Cleavage
- RNA Precursors/genetics
- Ribonuclease III/metabolism
- Transcription, Genetic