Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification

Saskia Neubacher, Christoph Arenz

Research output: Contribution to JournalArticleAcademic

Abstract

The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.

Original languageEnglish
Pages (from-to)109-19
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume1095
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • DNA Replication
  • DNA, Single-Stranded/metabolism
  • MicroRNAs/genetics
  • Nucleic Acid Amplification Techniques/methods
  • Phosphorylation
  • RNA Cleavage
  • RNA Precursors/genetics
  • Ribonuclease III/metabolism
  • Transcription, Genetic

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