Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification

Saskia Neubacher; Christoph Arenz

doi:10.1007/978-1-62703-703-7_9

Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification

Research output: Contribution to Journal › Article › Academic

Abstract

The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.

Original language	English
Pages (from-to)	109-19
Number of pages	11
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1095
DOIs	https://doi.org/10.1007/978-1-62703-703-7_9
Publication status	Published - 2014
Externally published	Yes

Keywords

DNA Replication
DNA, Single-Stranded/metabolism
MicroRNAs/genetics
Nucleic Acid Amplification Techniques/methods
Phosphorylation
RNA Cleavage
RNA Precursors/genetics
Ribonuclease III/metabolism
Transcription, Genetic

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title = "Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification",

abstract = "The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification. ",

keywords = "DNA Replication, DNA, Single-Stranded/metabolism, MicroRNAs/genetics, Nucleic Acid Amplification Techniques/methods, Phosphorylation, RNA Cleavage, RNA Precursors/genetics, Ribonuclease III/metabolism, Transcription, Genetic",

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year = "2014",

doi = "10.1007/978-1-62703-703-7_9",

language = "English",

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AU - Arenz, Christoph

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AB - The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.

KW - DNA Replication

KW - DNA, Single-Stranded/metabolism

KW - MicroRNAs/genetics

KW - Nucleic Acid Amplification Techniques/methods

KW - Phosphorylation

KW - RNA Cleavage

KW - RNA Precursors/genetics

KW - Ribonuclease III/metabolism

KW - Transcription, Genetic

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DO - 10.1007/978-1-62703-703-7_9

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EP - 119

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

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