Abstract
A reversed-phase ion-pair chromatographic method with on-line radioactivity detection for the simultaneous determination of 195mPt-labelled cisplatin and related platinum complexes has been developed. With this system a good resolution of various radiolabelled platinum complexes can be achieved. The detection limit of the radioactivity detector is 10 ng of cisplatin (specific activity of 15 MBq/mg cisplatin) per millilitre of urine or plasma ultrafiltrate. The detector response is independent of both the chemical structure of the platinum complexes and the matrix composition of the samples. This method may serve as a reference system for other high-performance liquid chromatographic systems with less specific and sensitive detectors.
| Original language | English |
|---|---|
| Pages (from-to) | 163-74 |
| Number of pages | 12 |
| Journal | Journal of Chromatography A |
| Volume | 491 |
| Issue number | 1 |
| Publication status | Published - 30 Jun 1989 |
Keywords
- Animals
- Chemical Phenomena
- Chemistry
- Chromatography, High Pressure Liquid
- Cisplatin
- Male
- Organoplatinum Compounds
- Radiometry
- Rats
- Rats, Inbred Strains
- Journal Article
- Research Support, Non-U.S. Gov't
