TY - JOUR
T1 - Determination of D- and L-Amino Acids in Biological Samples by Two Dimensional Column Liquid Chromatography.
AU - van de Merbel, N.C.
AU - Lingeman, H.
AU - Brinkman, U.A.T.
AU - Stenberg, M.
AU - Oste, R.
AU - Marko-Varga, G.
AU - Gorton, L.
PY - 1995
Y1 - 1995
N2 - A two-dimensional, column liquid chromatographic system is used for the determination of the D- and L-enantiomers of amino acids in biological samples. Separation of the amino acids is first on ion-exchange column by gradient elution with a sodium citrate-sodium chloride buffer. Enantioseparation is by subsequent injection of 3 μl heart-cuts of the individual amino acids onto a second column with a chiral crown ether stationary phase. Finally, fluorescence detection is after post-column labelling of the amino acids using an o-phthalaldehyde-2-mercaptoethanol reagent solution. The high separation power and selectivity of the system allow processing of complex samples with hardly any additional treatment and the determination of small quantities of D-amino acids in the presence of excess L-form. Applicability of the system is illustrated by the determination of D- and L-aspartate, serine, glutamate and alanine in various complex biological samples, such as protein hydrolysates, urine and biotechnological and food samples. Data are given on detectability, repeatability and linearity. © 1995 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
AB - A two-dimensional, column liquid chromatographic system is used for the determination of the D- and L-enantiomers of amino acids in biological samples. Separation of the amino acids is first on ion-exchange column by gradient elution with a sodium citrate-sodium chloride buffer. Enantioseparation is by subsequent injection of 3 μl heart-cuts of the individual amino acids onto a second column with a chiral crown ether stationary phase. Finally, fluorescence detection is after post-column labelling of the amino acids using an o-phthalaldehyde-2-mercaptoethanol reagent solution. The high separation power and selectivity of the system allow processing of complex samples with hardly any additional treatment and the determination of small quantities of D-amino acids in the presence of excess L-form. Applicability of the system is illustrated by the determination of D- and L-aspartate, serine, glutamate and alanine in various complex biological samples, such as protein hydrolysates, urine and biotechnological and food samples. Data are given on detectability, repeatability and linearity. © 1995 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
UR - https://www.scopus.com/pages/publications/51649137271
UR - https://www.scopus.com/inward/citedby.url?scp=51649137271&partnerID=8YFLogxK
U2 - 10.1007/BF02687993
DO - 10.1007/BF02687993
M3 - Article
SN - 0009-5893
VL - 41
SP - 6
JO - Chromatographia
JF - Chromatographia
ER -
