Determination of p-Methoxyphenacyl Bromide Derivatives of Alkylmethylphosphonic Acids in Urine Using Gas Chromatography with High-Resolution Mass Spectrometric Detection

D.V. Oreshkin, T.M. Baygildiev, M.F. Vokuev, A.V. Braun, I.A. Godovikov, I.V. Rybalchenko, I.A. Rodin

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

© 2021, Pleiades Publishing, Ltd.Abstract: We developed a method for determining ethyl methylphosphonic, isopropyl methylphosphonic, isobutyl methylphosphonic, and pinacolyl methylphosphonic acids in human urine using gas chromatography with high-resolution mass spectrometry and preliminary derivatization with p-methoxyphenacyl bromide. All of the acids are specific hydrolysis products and biomarkers of nerve agents. Conditions of the derivatization reaction were optimized. Features and general patterns of the MS1 and MS2 spectra of derivatives acquired in electron and chemical ionization modes are revealed. The developed method achieved the limits of detection in the range from 20 to 70 ng/mL.
Original languageEnglish
Pages (from-to)1530-1537
JournalJournal of Analytical Chemistry
Volume76
Issue number13
DOIs
Publication statusPublished - 1 Dec 2021
Externally publishedYes

Funding

The study was supported by the Russian Foundation for Basic Research, project no. 18-33-20068 mol_a_ved.

FundersFunder number
Russian Foundation for Basic Research18-33-20068

    Fingerprint

    Dive into the research topics of 'Determination of p-Methoxyphenacyl Bromide Derivatives of Alkylmethylphosphonic Acids in Urine Using Gas Chromatography with High-Resolution Mass Spectrometric Detection'. Together they form a unique fingerprint.

    Cite this