Determination of the anti-cancer drug metabolite WR1065 using pre-column derivatization and diode laser-induced fluorescence detection.

A.J.G. Mank, C. Gooijer, H. Lingeman, N.H. Velthorst, U.A.T. Brinkman, E.J. Molenaar

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 × 10
Original languageEnglish
Pages (from-to)255
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume13
DOIs
Publication statusPublished - 1995

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