TY - JOUR
T1 - Determination of the anti-cancer drug metabolite WR1065 using pre-column derivatization and diode laser-induced fluorescence detection.
AU - Mank, A.J.G.
AU - Gooijer, C.
AU - Lingeman, H.
AU - Velthorst, N.H.
AU - Brinkman, U.A.T.
AU - Molenaar, E.J.
PY - 1995
Y1 - 1995
N2 - A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 × 10
AB - A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 × 10
U2 - 10.1016/0731-7085(95)01286-T
DO - 10.1016/0731-7085(95)01286-T
M3 - Article
SN - 0731-7085
VL - 13
SP - 255
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -
