Determination of the anti-cancer drug metabolite WR1065 using pre-column derivatization and diode laser-induced fluorescence detection.

A.J.G. Mank; C. Gooijer; H. Lingeman; N.H. Velthorst; U.A.T. Brinkman; E.J. Molenaar

doi:10.1016/0731-7085(95)01286-T

Determination of the anti-cancer drug metabolite WR1065 using pre-column derivatization and diode laser-induced fluorescence detection.

A.J.G. Mank
, C. Gooijer
, H. Lingeman
, N.H. Velthorst
, U.A.T. Brinkman
, E.J. Molenaar

Chemistry and Pharmaceutical Sciences

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 × 10

Original language	English
Pages (from-to)	255
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	13
DOIs	https://doi.org/10.1016/0731-7085(95)01286-T
Publication status	Published - 1995

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10.1016/0731-7085(95)01286-T

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title = "Determination of the anti-cancer drug metabolite WR1065 using pre-column derivatization and diode laser-induced fluorescence detection.",

abstract = "A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 × 10",

author = "A.J.G. Mank and C. Gooijer and H. Lingeman and N.H. Velthorst and U.A.T. Brinkman and E.J. Molenaar",

year = "1995",

doi = "10.1016/0731-7085(95)01286-T",

language = "English",

volume = "13",

pages = "255",

journal = "Journal of Pharmaceutical and Biomedical Analysis",

issn = "0731-7085",

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TY - JOUR

T1 - Determination of the anti-cancer drug metabolite WR1065 using pre-column derivatization and diode laser-induced fluorescence detection.

AU - Mank, A.J.G.

AU - Gooijer, C.

AU - Lingeman, H.

AU - Velthorst, N.H.

AU - Brinkman, U.A.T.

AU - Molenaar, E.J.

PY - 1995

Y1 - 1995

N2 - A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 × 10

AB - A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 × 10

U2 - 10.1016/0731-7085(95)01286-T

DO - 10.1016/0731-7085(95)01286-T

M3 - Article

SN - 0731-7085

VL - 13

SP - 255

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

ER -

Determination of the anti-cancer drug metabolite WR1065 using pre-column derivatization and diode laser-induced fluorescence detection.

Abstract

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