Development and Validation of a CE Method for the Determination of Tetracyclines with Capacitively Coupled Contactless Conductivity Detection

Prasanta Paul, Josephine Reynaert, Cari Sänger-van de Griend, Erwin Adams, Ann Van Schepdael*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In this study, a simple and robust capillary electrophoresis method with capacitively coupled contactless conductivity detection (C4D) is developed for the determination of the tetracycline antibiotics (1) tetracycline, (2) chlortetracycline and (3) oxytetracycline. An uncoated, fused silica capillary (60.2 cm long, 75 µm i.d.) and a solution of 50 mM tris(hydroxymethyl) aminomethane, 50 mM l-histidine and 5 mM methyl-β-cyclodextrin, without pH adjustment (pH 8.76), was used as background electrolyte. Electrophoresis at + 25 kV showed a rapid analysis with sufficient resolution among the three antibiotics in the order of tetracycline, chlortetracycline and oxytetracycline. Successive inter-injection rinsing (20 psi) of the capillary ensured intra- and inter-day repeatability (0.9–2.2% RSD and 2.0–4.5% RSD, respectively, for relative peak areas). The method showed satisfactory performance in terms of selectivity, accuracy (99.3–101.4%) and linearity (R2 = 0.999). Finally, the method was applied to commercial samples of tetracycline, oxytetracycline and chlortetracycline. This method can be applied for rapid quality control in developing countries in particular, and across the globe in general.

Original languageEnglish
Pages (from-to)1395-1403
Number of pages9
JournalChromatographia
Volume82
Issue number9
DOIs
Publication statusPublished - 1 Sep 2019
Externally publishedYes

Keywords

  • Capacitively coupled contactless conductivity detection
  • Capillary electrophoresis
  • Tetracycline antibiotics
  • Validation

Fingerprint

Dive into the research topics of 'Development and Validation of a CE Method for the Determination of Tetracyclines with Capacitively Coupled Contactless Conductivity Detection'. Together they form a unique fingerprint.

Cite this