Development and validation of a fluorescence HPLC-based screening assay for inhibition of human estrogen sulfotransferase

J. Reinen; E. Vriese; H.R. Glatt; N.P.E. Vermeulen

doi:10.1016/j.ab.2006.07.015

Development and validation of a fluorescence HPLC-based screening assay for inhibition of human estrogen sulfotransferase

J. Reinen, E. Vriese, H.R. Glatt, N.P.E. Vermeulen

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Human estrogen sulfotransferase (SULT1E1) is involved in the regulation of 17β-estradiol responsiveness and is believed to protect peripheral tissues from excessive estrogenic effects. Several assays already have been developed to investigate the inhibitory effect of endocrine-disrupting compounds (EDCs) on SULT1E1. However, most of these assays make use of the radiolabeled cofactor [

Original language	English
Pages (from-to)	85-92
Journal	Analytical Biochemistry
Volume	357
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2006.07.015
Publication status	Published - 2006

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10.1016/j.ab.2006.07.015

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title = "Development and validation of a fluorescence HPLC-based screening assay for inhibition of human estrogen sulfotransferase",

abstract = "Human estrogen sulfotransferase (SULT1E1) is involved in the regulation of 17β-estradiol responsiveness and is believed to protect peripheral tissues from excessive estrogenic effects. Several assays already have been developed to investigate the inhibitory effect of endocrine-disrupting compounds (EDCs) on SULT1E1. However, most of these assays make use of the radiolabeled cofactor [",

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AU - Vermeulen, N.P.E.

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AB - Human estrogen sulfotransferase (SULT1E1) is involved in the regulation of 17β-estradiol responsiveness and is believed to protect peripheral tissues from excessive estrogenic effects. Several assays already have been developed to investigate the inhibitory effect of endocrine-disrupting compounds (EDCs) on SULT1E1. However, most of these assays make use of the radiolabeled cofactor [

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DO - 10.1016/j.ab.2006.07.015

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Development and validation of a fluorescence HPLC-based screening assay for inhibition of human estrogen sulfotransferase

Abstract

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