Development of a profiling strategy for metabolic mixtures by combining chromatography and mass spectrometry with cell-based GPCR signaling.

S. Nijmeijer, H.F. Vischer, A.F. Rudebeck, F Fleurbaaij, D. Falck, R. Leurs, W.M.A. Niessen, J. Kool

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In this study, we developed an in-line methodology that combines analytical with pharmacological techniques to characterize metabolites of human histamine H4 receptor (hH4R) ligands. Liquid chromatographic separation of metabolic mixtures is coupled to high-resolution fractionation into 96- or 384-well plates and directly followed by a cell-based reporter gene assay to measure receptor signaling. The complete methodology was designed, optimized, validated, and ultimately miniaturized into a high-density well plate format. Finally, the methodology was demonstrated in a metabolic profiling setting for three hH4R lead compounds and the drug clozapine. This new methodology comprises integrated analytical separations, mass spectrometry, and a cell-based signal transduction-driven reporter gene assay that enables the implementation of comprehensive metabolic profiling earlier in the drug discovery process. © 2012 Society for Laboratory Automation and Screening.
Original languageEnglish
Pages (from-to)1329-1338
JournalJournal of Biomolecular Screening
Volume17
DOIs
Publication statusPublished - 2012

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