Development of a proxy forpast surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores.

P. Blokker; D. Yeloff; P. Boelen

doi:10.1021/ac050696k

Development of a proxy forpast surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores.

P. Blokker, D. Yeloff, P. Boelen

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectromtry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of ∼60 fresh pollen of Alnus glutinosa and a relative standard deviation of ∼10% between 100 and 600 pollen. © 2005 American Chemical Society.

Original language	English
Pages (from-to)	6026-6031
Journal	Analytical Chemistry
Volume	77
Issue number	18
DOIs	https://doi.org/10.1021/ac050696k
Publication status	Published - 2005

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title = "Development of a proxy forpast surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores.",

abstract = "A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectromtry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of ∼60 fresh pollen of Alnus glutinosa and a relative standard deviation of ∼10% between 100 and 600 pollen. {\textcopyright} 2005 American Chemical Society.",

author = "P. Blokker and D. Yeloff and P. Boelen",

year = "2005",

doi = "10.1021/ac050696k",

language = "English",

volume = "77",

pages = "6026--6031",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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T1 - Development of a proxy forpast surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores.

AU - Blokker, P.

AU - Yeloff, D.

AU - Boelen, P.

PY - 2005

Y1 - 2005

N2 - A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectromtry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of ∼60 fresh pollen of Alnus glutinosa and a relative standard deviation of ∼10% between 100 and 600 pollen. © 2005 American Chemical Society.

AB - A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectromtry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of ∼60 fresh pollen of Alnus glutinosa and a relative standard deviation of ∼10% between 100 and 600 pollen. © 2005 American Chemical Society.

U2 - 10.1021/ac050696k

DO - 10.1021/ac050696k

M3 - Article

SN - 0003-2700

VL - 77

SP - 6026

EP - 6031

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 18

ER -

Development of a proxy forpast surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores.

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