Development of a surface plasmon resonance sensor for coupling to capillary electrophoresis allowing affinity assessment of protein mixture components

Elena Domínguez-Vega*, Rob Haselberg, Dick van Iperen, Jeroen Kool, Govert W. Somsen, Gerhardus J. de Jong

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

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Abstract

Surface plasmon resonance (SPR) currently is the major platform to study protein–protein interactions, but it lacks the selectivity to distinguish between binding components within one sample. Capillary electrophoresis (CE) can provide efficient separation of intact proteins under near-physiological conditions. We have hyphenated CE with SPR to achieve affinity assessment of mixture components. A microfluidic flow cell allowing straightforward coupling of CE and SPR was developed. Initial testing with non-interacting dyes showed good performance using a flow-cell channel volume of 100 nL until the detection point. Appropriate closing of the CE electric circuit was achieved using the SPR gold-sensor as grounding electrode. Division of the (bio)sensor into an electrode part (providing grounding) and a detection part (bearing the affinity surface) was crucial to avoid disturbance of the SPR signal by the CE voltage. This approach permitted CE separation and binding assessment for separation voltages up to 30 kV. Human serum albumin (HSA) or aprotinin were immobilized on carboxymethyldextran hydrogel-coated gold sensors and target proteins (anti-HSA, and trypsin and α-chymotrypsin, respectively) were analyzed. Efficient CE separation of the intact protein analytes was accomplished under native conditions by employing neutral and positively-charged capillary coatings. Selective binding of separated proteins to the target surface could be monitored by SPR down to 2 ng of injected protein. Regeneration of the biosensor surface was achieved by an on-line rising, allowing repeatable CE-SPR analyses of proteins with RSDs below 1% and 5% for migration time and signal intensity, respectively.

Original languageEnglish
Pages (from-to)1040-1047
Number of pages8
JournalSensors and Actuators B-Chemical
Volume254
Early online date30 Jul 2017
DOIs
Publication statusPublished - Jan 2018

Funding

This work was supported by the Dutch Technology Foundation STW, which is part of The Netherlands Organisation for Scientific Research (NWO) and partly funded by the Ministry of Economic Affairs (grant number 11056). The authors would like to thank Bionavis (Tampere, Finland) for making available a multiparametric SPR Navi 210A instrument. Furthermore, we would in particular like to thank Jussi Tuppurainen and Johana Kuncova-Kallio from Bionavis for helpful discussions and their input.

FundersFunder number
Ministerie van Economische Zaken11056
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Stichting voor de Technische Wetenschappen

    Keywords

    • Biosensor
    • Capillary electrophoresis
    • Protein-protein interactions
    • Selective binding
    • SPR

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