Development of an alternative artificial soil for earthworm toxicity testing in tropical countries.

M. de Silva, C.A.M. van Gestel

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The standard soil invertebrate toxicity tests developed by OECD and ISO use an artificial soil as the test substrate, which contains sphagnum peat as a component. This type of peat is not widely available. Investigation of possible alternative substrates using locally available materials therefore is vital for performing such ecotoxicity tests, particularly in the tropics. We studied the suitability of paddy husk (PH), saw-dust (SD), non-composted (NCCP) and composted coco peat (CCP) as a replacement for sphagnum peat. Artificial soil (AS) was prepared by mixing 70% sand and 20% kaolin clay with 10% PH, SD, NCCP or CCP. First, the reproduction potential of the earthworm Eisenia andrei was investigated in modified artificial soil (MAS) using the original OECD AS as the control. The number of juveniles produced in OECD AS, MAS
Original languageEnglish
Pages (from-to)170-174
JournalApplied Soil Ecology
Volume43
DOIs
Publication statusPublished - 2009

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