Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents

J. Carol-Visser, M. van der Schans, A. Fidder, A.G. Huist, B.L.M. van Baar, H. Irth, D. Noort

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. © 2008 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)91-97
JournalJournal of Chromatography B
Volume870
Issue number1
DOIs
Publication statusPublished - 2008

Fingerprint

Chemical Warfare Agents
Pepsin A
Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Digestion
Mass Spectrometry
Sarin
Mustard Gas
Proteins
Monitoring
Cholinesterases
Serum Albumin

Cite this

Carol-Visser, J. ; van der Schans, M. ; Fidder, A. ; Huist, A.G. ; van Baar, B.L.M. ; Irth, H. ; Noort, D. / Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents. In: Journal of Chromatography B. 2008 ; Vol. 870, No. 1. pp. 91-97.
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abstract = "Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. {\circledC} 2008 Elsevier B.V. All rights reserved.",
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Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents. / Carol-Visser, J.; van der Schans, M.; Fidder, A.; Huist, A.G.; van Baar, B.L.M.; Irth, H.; Noort, D.

In: Journal of Chromatography B, Vol. 870, No. 1, 2008, p. 91-97.

Research output: Contribution to JournalArticleAcademicpeer-review

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AB - Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. © 2008 Elsevier B.V. All rights reserved.

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