Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents

J. Carol-Visser; M. van der Schans; A. Fidder; A.G. Huist; B.L.M. van Baar; H. Irth; D. Noort

doi:10.1016/j.jchromb.2008.06.008

Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents

J. Carol-Visser, M. van der Schans, A. Fidder, A.G. Huist, B.L.M. van Baar, H. Irth, D. Noort

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. © 2008 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	91-97
Journal	Journal of Chromatography B
Volume	870
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2008.06.008
Publication status	Published - 2008

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10.1016/j.jchromb.2008.06.008

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Carol-Visser, J., van der Schans, M., Fidder, A., Huist, A. G., van Baar, B. L. M., Irth, H., & Noort, D. (2008). Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents. Journal of Chromatography B, 870(1), 91-97. https://doi.org/10.1016/j.jchromb.2008.06.008

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title = "Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents",

abstract = "Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. {\textcopyright} 2008 Elsevier B.V. All rights reserved.",

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year = "2008",

doi = "10.1016/j.jchromb.2008.06.008",

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Carol-Visser, J, van der Schans, M, Fidder, A, Huist, AG, van Baar, BLM, Irth, H & Noort, D 2008, 'Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents', Journal of Chromatography B, vol. 870, no. 1, pp. 91-97. https://doi.org/10.1016/j.jchromb.2008.06.008

Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents. / Carol-Visser, J.; van der Schans, M.; Fidder, A. et al.
In: Journal of Chromatography B, Vol. 870, No. 1, 2008, p. 91-97.

Research output: Contribution to Journal › Article › Academic › peer-review

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AU - Carol-Visser, J.

AU - van der Schans, M.

AU - Fidder, A.

AU - Huist, A.G.

AU - van Baar, B.L.M.

AU - Irth, H.

AU - Noort, D.

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AB - Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. © 2008 Elsevier B.V. All rights reserved.

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Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents

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