Developmental exposure to triclosan and benzophenone-2 causes morphological alterations in zebrafish (Danio rerio) thyroid follicles and eyes

Maximilian Kraft; Lisa Gölz; Maximilian Rinderknecht; Johannes Koegst; Thomas Braunbeck; Lisa Baumann

doi:10.1007/s11356-022-24531-2

Developmental exposure to triclosan and benzophenone-2 causes morphological alterations in zebrafish (Danio rerio) thyroid follicles and eyes

Maximilian Kraft, Lisa Gölz, Maximilian Rinderknecht, Johannes Koegst, Thomas Braunbeck, Lisa Baumann

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Thyroid hormones (THs) regulate a multitude of developmental and metabolic processes, which are responsible for vertebrate development, growth, and maintenance of homeostasis. THs also play a key role in neurogenesis of vertebrates and thus affect eye development, which is vital for foraging efficiency and for effective escape from predation. Currently, there are no validated test guidelines for the assessment of TH system-disrupting chemicals (THSDCs) in fish. Consequently, the present study was designed to demonstrate the suitability of novel thyroid-related endpoints in early life-stages of fish. Embryos of a transgenic zebrafish (Danio rerio) line expressing the reporter gene tg:mCherry in their thyrocytes were used to investigate the effects of the environmental THSDCs triclosan (TCS, antibacterial agent) and benzophenone-2 (BP-2, UV filter) on thyroid follicle and eye development. Both BP-2 and TCS caused thyroid follicle hyperplasia in transgenic zebrafish, thus confirming their role as THSDCs. The effect intensity on follicle size and fluorescence was comparable with a 1.7-fold increase for BP-2 and 1.6-fold for TCS. Alterations of the cellular structures of the retina indicate an impact of both substances on eye development, with a stronger impact of TCS. With respect to guideline development, results provide further evidence for the suitability of morphological changes in thyroid follicles and the eyes as novel endpoints for the sensitive assessment of THSD-related effects in fish.

Original language	English
Pages (from-to)	33711-33724
Number of pages	14
Journal	Environmental science and pollution research international
Volume	30
Issue number	12
DOIs	https://doi.org/10.1007/s11356-022-24531-2
Publication status	Published - Mar 2023

Bibliographical note

Funding

Open Access funding enabled and organized by Projekt DEAL. This study received funding from the European Union’s Horizon 2020 research and innovation programme, under grant agreement No. 825753 (ERGO). LB received funding from the “Olympia Morata Programme” of the Office of Equal Opportunities, University of Heidelberg.

Funders	Funder number
Horizon 2020 Framework Programme	825753
Universität Heidelberg

Keywords

Animals
Triclosan/toxicity
Zebrafish/genetics
Thyroid Gland
Benzophenones/toxicity
Thyroid Hormones/metabolism
Water Pollutants, Chemical/metabolism

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10.1007/s11356-022-24531-2

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@article{707aae59b7b84c679f4a7050b6954366,

title = "Developmental exposure to triclosan and benzophenone-2 causes morphological alterations in zebrafish (Danio rerio) thyroid follicles and eyes",

abstract = "Thyroid hormones (THs) regulate a multitude of developmental and metabolic processes, which are responsible for vertebrate development, growth, and maintenance of homeostasis. THs also play a key role in neurogenesis of vertebrates and thus affect eye development, which is vital for foraging efficiency and for effective escape from predation. Currently, there are no validated test guidelines for the assessment of TH system-disrupting chemicals (THSDCs) in fish. Consequently, the present study was designed to demonstrate the suitability of novel thyroid-related endpoints in early life-stages of fish. Embryos of a transgenic zebrafish (Danio rerio) line expressing the reporter gene tg:mCherry in their thyrocytes were used to investigate the effects of the environmental THSDCs triclosan (TCS, antibacterial agent) and benzophenone-2 (BP-2, UV filter) on thyroid follicle and eye development. Both BP-2 and TCS caused thyroid follicle hyperplasia in transgenic zebrafish, thus confirming their role as THSDCs. The effect intensity on follicle size and fluorescence was comparable with a 1.7-fold increase for BP-2 and 1.6-fold for TCS. Alterations of the cellular structures of the retina indicate an impact of both substances on eye development, with a stronger impact of TCS. With respect to guideline development, results provide further evidence for the suitability of morphological changes in thyroid follicles and the eyes as novel endpoints for the sensitive assessment of THSD-related effects in fish.",

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language = "English",

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Developmental exposure to triclosan and benzophenone-2 causes morphological alterations in zebrafish (Danio rerio) thyroid follicles and eyes. / Kraft, Maximilian; Gölz, Lisa; Rinderknecht, Maximilian et al.
In: Environmental science and pollution research international, Vol. 30, No. 12, 03.2023, p. 33711-33724.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Developmental exposure to triclosan and benzophenone-2 causes morphological alterations in zebrafish (Danio rerio) thyroid follicles and eyes

AU - Kraft, Maximilian

AU - Gölz, Lisa

AU - Rinderknecht, Maximilian

AU - Koegst, Johannes

AU - Braunbeck, Thomas

AU - Baumann, Lisa

PY - 2023/3

Y1 - 2023/3

N2 - Thyroid hormones (THs) regulate a multitude of developmental and metabolic processes, which are responsible for vertebrate development, growth, and maintenance of homeostasis. THs also play a key role in neurogenesis of vertebrates and thus affect eye development, which is vital for foraging efficiency and for effective escape from predation. Currently, there are no validated test guidelines for the assessment of TH system-disrupting chemicals (THSDCs) in fish. Consequently, the present study was designed to demonstrate the suitability of novel thyroid-related endpoints in early life-stages of fish. Embryos of a transgenic zebrafish (Danio rerio) line expressing the reporter gene tg:mCherry in their thyrocytes were used to investigate the effects of the environmental THSDCs triclosan (TCS, antibacterial agent) and benzophenone-2 (BP-2, UV filter) on thyroid follicle and eye development. Both BP-2 and TCS caused thyroid follicle hyperplasia in transgenic zebrafish, thus confirming their role as THSDCs. The effect intensity on follicle size and fluorescence was comparable with a 1.7-fold increase for BP-2 and 1.6-fold for TCS. Alterations of the cellular structures of the retina indicate an impact of both substances on eye development, with a stronger impact of TCS. With respect to guideline development, results provide further evidence for the suitability of morphological changes in thyroid follicles and the eyes as novel endpoints for the sensitive assessment of THSD-related effects in fish.

AB - Thyroid hormones (THs) regulate a multitude of developmental and metabolic processes, which are responsible for vertebrate development, growth, and maintenance of homeostasis. THs also play a key role in neurogenesis of vertebrates and thus affect eye development, which is vital for foraging efficiency and for effective escape from predation. Currently, there are no validated test guidelines for the assessment of TH system-disrupting chemicals (THSDCs) in fish. Consequently, the present study was designed to demonstrate the suitability of novel thyroid-related endpoints in early life-stages of fish. Embryos of a transgenic zebrafish (Danio rerio) line expressing the reporter gene tg:mCherry in their thyrocytes were used to investigate the effects of the environmental THSDCs triclosan (TCS, antibacterial agent) and benzophenone-2 (BP-2, UV filter) on thyroid follicle and eye development. Both BP-2 and TCS caused thyroid follicle hyperplasia in transgenic zebrafish, thus confirming their role as THSDCs. The effect intensity on follicle size and fluorescence was comparable with a 1.7-fold increase for BP-2 and 1.6-fold for TCS. Alterations of the cellular structures of the retina indicate an impact of both substances on eye development, with a stronger impact of TCS. With respect to guideline development, results provide further evidence for the suitability of morphological changes in thyroid follicles and the eyes as novel endpoints for the sensitive assessment of THSD-related effects in fish.

KW - Animals

KW - Triclosan/toxicity

KW - Zebrafish/genetics

KW - Thyroid Gland

KW - Benzophenones/toxicity

KW - Thyroid Hormones/metabolism

KW - Water Pollutants, Chemical/metabolism

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UR - https://www.scopus.com/inward/citedby.url?scp=85143583839&partnerID=8YFLogxK

U2 - 10.1007/s11356-022-24531-2

DO - 10.1007/s11356-022-24531-2

M3 - Article

C2 - 36495432

SN - 0944-1344

VL - 30

SP - 33711

EP - 33724

JO - Environmental science and pollution research international

JF - Environmental science and pollution research international

IS - 12

ER -

Developmental exposure to triclosan and benzophenone-2 causes morphological alterations in zebrafish (Danio rerio) thyroid follicles and eyes

Abstract

Bibliographical note

Funding

Keywords

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