Diazirine based photoaffinity labeling

Luba Dubinsky; Bastiaan P Krom; Michael M Meijler

doi:10.1016/j.bmc.2011.06.066

Diazirine based photoaffinity labeling

Luba Dubinsky, Bastiaan P Krom, Michael M Meijler

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Research output: Contribution to Journal › Review article › Academic › peer-review

Abstract

Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

Original language	English
Pages (from-to)	554-70
Number of pages	17
Journal	Bioorganic and Medicinal Chemistry
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/j.bmc.2011.06.066
Publication status	Published - 15 Jan 2012

Bibliographical note

Keywords

Diazomethane/chemical synthesis
Enzymes/chemistry
Nucleic Acids/chemistry
Pharmaceutical Preparations/chemistry
Photoaffinity Labels/chemistry
Protein Interaction Mapping
Proteins/chemistry

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10.1016/j.bmc.2011.06.066

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title = "Diazirine based photoaffinity labeling",

abstract = "Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.",

keywords = "Diazomethane/chemical synthesis, Enzymes/chemistry, Nucleic Acids/chemistry, Pharmaceutical Preparations/chemistry, Photoaffinity Labels/chemistry, Protein Interaction Mapping, Proteins/chemistry",

author = "Luba Dubinsky and Krom, {Bastiaan P} and Meijler, {Michael M}",

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AU - Dubinsky, Luba

AU - Krom, Bastiaan P

AU - Meijler, Michael M

PY - 2012/1/15

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N2 - Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

AB - Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

KW - Diazomethane/chemical synthesis

KW - Enzymes/chemistry

KW - Nucleic Acids/chemistry

KW - Pharmaceutical Preparations/chemistry

KW - Photoaffinity Labels/chemistry

KW - Protein Interaction Mapping

KW - Proteins/chemistry

U2 - 10.1016/j.bmc.2011.06.066

DO - 10.1016/j.bmc.2011.06.066

M3 - Review article

C2 - 21778062

SN - 0968-0896

VL - 20

SP - 554

EP - 570

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

IS - 2

ER -

Diazirine based photoaffinity labeling

Abstract

Bibliographical note

Keywords

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