Diffusion of antimicrobials in multispecies biofilms evaluated in a new biofilm model

S.V. van der Waal; J. de Almeida; B.P. Krom; J.J. de Soet; W. Crielaard

doi:10.1111/iej.12634

Diffusion of antimicrobials in multispecies biofilms evaluated in a new biofilm model

S.V. van der Waal
, J. de Almeida
, B.P. Krom
, J.J. de Soet
, W. Crielaard

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Aim: To describe the application of a newly-developed in vitro model in which the diffusion of antimicrobials in oral biofilms can be studied.

Methodology: In a flow chamber consisting of three parallel feeding channels connected with each other by eight perpendicular side channels, multispecies biofilms were grown from saliva of a single donor for 48 h. The dimensions of the side channels were 100 μm × 100 μm × 5130 μm (H × W × L). When one or more side channels were filled with biofilm, the biofilms were stained with fluorescent stains. Then, one side-channel biofilm was selected and treated with phosphate buffered saline, 2% sodium hypochlorite (NaOCl), 17% ethylenediaminetetra-acetic acid (EDTA) or modified salt solution (MSS). Diffusion of the irrigants was observed by acquiring fluorescence images at 10× objective every 15 s for 30 min.

Results: It was possible to culture biofilms in the narrow (100 μm) channels. The biofilms varied in phenotype. In this model, no diffusion of NaOCl into the biofilms was seen after its application. Seventeen-percentage EDTA only diffused into the biofilm up to 200 μm in 30 min. MSS did diffuse in the biofilm over a distance of 450 μm within 2 min after a single application.

Conclusions: This new model enables the investigation of the diffusion of antimicrobials in biofilms. Other applications to improve our understanding of the characteristics of biofilms are now possible.

Original language	English
Pages (from-to)	367-376
Journal	International Endodontic Journal
Volume	50
Issue number	4
Early online date	19 Mar 2016
DOIs	https://doi.org/10.1111/iej.12634
Publication status	Published - Apr 2017

Funding

This study has been partly funded by the annual research grant of the European Society of Endodontology and the research grant of the Netherlands Society of Endodontology. Financial assistance from CAPES (Process number 11 842/13-0) which enabled Josiane de Almeida to hold a Visiting Research Fellow at ACTA – Academic Centre Dentistry Amsterdam is graciously acknowledged. This research was also partially funded by NWO Earth and Life Sciences (ALW), project 834.13.006. Two authors, SVvdW and JJdS, are co-inventors of the intellectual property which is described in the pending patent: Composition, used e.g. as disinfectant or antimicrobial in dental treatment (e.g. root canal treatments), comprises at least one tonicity agent (organic acids and/or their salts e.g. sodium acetate) rendering the composition hypertonic. The patent is owned by University of Amsterdam, Amsterdam, Netherlands. Patent Numbers: WO2011102724-A2; NL2004260-C; WO2011102724-A3; US3012328708-A1; EP2536378-A2; CN102985051-A.

Funders	Funder number
Josiane de Almeida
Netherlands Society of Endodontology
European Society of Endocrinology
Universiteit van Amsterdam	US3012328708-A1, WO2011102724-A2, WO2011102724-A3, EP2536378-A2
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	11 842/13-0
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	834.13.006

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

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title = "Diffusion of antimicrobials in multispecies biofilms evaluated in a new biofilm model",

abstract = "Aim: To describe the application of a newly-developed in vitro model in which the diffusion of antimicrobials in oral biofilms can be studied. Methodology: In a flow chamber consisting of three parallel feeding channels connected with each other by eight perpendicular side channels, multispecies biofilms were grown from saliva of a single donor for 48 h. The dimensions of the side channels were 100 μm × 100 μm × 5130 μm (H × W × L). When one or more side channels were filled with biofilm, the biofilms were stained with fluorescent stains. Then, one side-channel biofilm was selected and treated with phosphate buffered saline, 2\% sodium hypochlorite (NaOCl), 17\% ethylenediaminetetra-acetic acid (EDTA) or modified salt solution (MSS). Diffusion of the irrigants was observed by acquiring fluorescence images at 10× objective every 15 s for 30 min. Results: It was possible to culture biofilms in the narrow (100 μm) channels. The biofilms varied in phenotype. In this model, no diffusion of NaOCl into the biofilms was seen after its application. Seventeen-percentage EDTA only diffused into the biofilm up to 200 μm in 30 min. MSS did diffuse in the biofilm over a distance of 450 μm within 2 min after a single application. Conclusions: This new model enables the investigation of the diffusion of antimicrobials in biofilms. Other applications to improve our understanding of the characteristics of biofilms are now possible.",

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doi = "10.1111/iej.12634",

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AU - van der Waal, S.V.

AU - de Almeida, J.

AU - Krom, B.P.

AU - de Soet, J.J.

AU - Crielaard, W.

PY - 2017/4

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N2 - Aim: To describe the application of a newly-developed in vitro model in which the diffusion of antimicrobials in oral biofilms can be studied. Methodology: In a flow chamber consisting of three parallel feeding channels connected with each other by eight perpendicular side channels, multispecies biofilms were grown from saliva of a single donor for 48 h. The dimensions of the side channels were 100 μm × 100 μm × 5130 μm (H × W × L). When one or more side channels were filled with biofilm, the biofilms were stained with fluorescent stains. Then, one side-channel biofilm was selected and treated with phosphate buffered saline, 2% sodium hypochlorite (NaOCl), 17% ethylenediaminetetra-acetic acid (EDTA) or modified salt solution (MSS). Diffusion of the irrigants was observed by acquiring fluorescence images at 10× objective every 15 s for 30 min. Results: It was possible to culture biofilms in the narrow (100 μm) channels. The biofilms varied in phenotype. In this model, no diffusion of NaOCl into the biofilms was seen after its application. Seventeen-percentage EDTA only diffused into the biofilm up to 200 μm in 30 min. MSS did diffuse in the biofilm over a distance of 450 μm within 2 min after a single application. Conclusions: This new model enables the investigation of the diffusion of antimicrobials in biofilms. Other applications to improve our understanding of the characteristics of biofilms are now possible.

AB - Aim: To describe the application of a newly-developed in vitro model in which the diffusion of antimicrobials in oral biofilms can be studied. Methodology: In a flow chamber consisting of three parallel feeding channels connected with each other by eight perpendicular side channels, multispecies biofilms were grown from saliva of a single donor for 48 h. The dimensions of the side channels were 100 μm × 100 μm × 5130 μm (H × W × L). When one or more side channels were filled with biofilm, the biofilms were stained with fluorescent stains. Then, one side-channel biofilm was selected and treated with phosphate buffered saline, 2% sodium hypochlorite (NaOCl), 17% ethylenediaminetetra-acetic acid (EDTA) or modified salt solution (MSS). Diffusion of the irrigants was observed by acquiring fluorescence images at 10× objective every 15 s for 30 min. Results: It was possible to culture biofilms in the narrow (100 μm) channels. The biofilms varied in phenotype. In this model, no diffusion of NaOCl into the biofilms was seen after its application. Seventeen-percentage EDTA only diffused into the biofilm up to 200 μm in 30 min. MSS did diffuse in the biofilm over a distance of 450 μm within 2 min after a single application. Conclusions: This new model enables the investigation of the diffusion of antimicrobials in biofilms. Other applications to improve our understanding of the characteristics of biofilms are now possible.

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Diffusion of antimicrobials in multispecies biofilms evaluated in a new biofilm model

Abstract

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UN SDGs

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