'Dilute-and-shoot' RSLC-MS-MS method for fast detection of nerve and vesicant chemical warfare agent metabolites in urine

I. Rodin, A. Braun, A. Stavrianidi, T. Baygildiev, O. Shpigun, D. Oreshkin, I. Rybalchenko

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

© The Author 2014.A sensitive screening method based on fast liquid chromatography tandem mass-spectrometry (RSLC-MS-MS) has shown the feasibility of separation and detection of low concentration β-lyase metabolites of sulfur mustard and of nerve agent phosphonic acids in urine. The analysis of these compounds is of interest because they are specific metabolites of the chemical warfare agents (CWAs), sulfur mustard (HD), sarin (GB), soman (GD), VX and Russian VX (RVX). The 'dilute-and-shoot' RSLC-MS-MS method provides a sensitive and direct approach for determining CWA exposure in non-extracted non-derivatized samples from urine. Chromatographic separation of the metabolites was achieved using a reverse phase column with gradient mobile phases consisting of 0.5% formic acid in water and acetonitrile. Identification and quantification of species were achieved using electrospray ionization-tandem mass-spectrometry monitoring two precursor-to-product ion transitions for each compound. The method demonstrates linearity over at least two orders of magnitude and had detection limits of 0.5 ng/mL in urine.
Original languageEnglish
Article numberbku119
Pages (from-to)69-74
JournalJournal of Analytical Toxicology
Volume39
Issue number1
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

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