TY - JOUR
T1 - Diode laser-induced fluorecscence detection in capillary electrophoresis after pre-column derivatization of amino acids and small peptides.
AU - Mank, A.J.G.
AU - Yeung, E.S.
PY - 1995
Y1 - 1995
N2 - The use of diode laser-induced fluorescence (DIO-LIF) detection in the field of capillary electrophoresis (CE) is examined. A simple but sensitive detection system was constructed. The performance of the system was evaluated with respect to design factors and its sensitivity was compared with the theoretically achievable sensitivity. To enhance the applicability of direct DIO-LIF detection in CE, a derivatization method for amines was developed. A red-absorbing label, consisting of a dicarbocyanine fluorophore with a succinimidyl ester functionality, was synthesized for this purpose. After derivatization of 1 × 10
AB - The use of diode laser-induced fluorescence (DIO-LIF) detection in the field of capillary electrophoresis (CE) is examined. A simple but sensitive detection system was constructed. The performance of the system was evaluated with respect to design factors and its sensitivity was compared with the theoretically achievable sensitivity. To enhance the applicability of direct DIO-LIF detection in CE, a derivatization method for amines was developed. A red-absorbing label, consisting of a dicarbocyanine fluorophore with a succinimidyl ester functionality, was synthesized for this purpose. After derivatization of 1 × 10
UR - https://www.scopus.com/pages/publications/0029077163
UR - https://www.scopus.com/inward/citedby.url?scp=0029077163&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(95)00418-M
DO - 10.1016/0021-9673(95)00418-M
M3 - Article
SN - 0021-9673
VL - 708
SP - 309
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -
