Direct observation of nanomechanical properties of chromatin in living cells

Anthony H B de Vries; Bea E Krenn; Roel van Driel; Vinod Subramaniam; Johannes S Kanger

doi:10.1021/nl070603+

Direct observation of nanomechanical properties of chromatin in living cells

Anthony H B de Vries, Bea E Krenn, Roel van Driel, Vinod Subramaniam, Johannes S Kanger

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Precise manipulation of nanometer-sized magnetic particles using magnetic tweezers has yielded insights into the rheology of the cell cytoplasm. We present first results using this approach to study the nanomechanics of the cell nucleus. Using a custom-designed micro-magnetic-tweezers instrument, we can achieve sufficiently high magnetic forces enabling the application and measurement of controlled distortion of the internal nuclear structure on the nanometer scale. We precisely measure the elasticity and viscosity inside the nucleus of living HeLa cells. The high value of the Young's modulus (Y = 2.5 x 10(2) Pa) measured relative to the cytoplasm is explained by a large-scale model for in vivo chromatin structure using a polymer network model.

Original language	English
Pages (from-to)	1424-7
Number of pages	4
Journal	Nano Letters
Volume	7
Issue number	5
DOIs	https://doi.org/10.1021/nl070603+
Publication status	Published - May 2007

Keywords

Chromatin
HeLa Cells
Humans
Nanotechnology
Journal Article
Research Support, Non-U.S. Gov't

Access to Document

10.1021/nl070603+

Persistent URL (handle)

Copy link address

Cite this

@article{3463974d282644ad89b96c0a31c92157,

title = "Direct observation of nanomechanical properties of chromatin in living cells",

abstract = "Precise manipulation of nanometer-sized magnetic particles using magnetic tweezers has yielded insights into the rheology of the cell cytoplasm. We present first results using this approach to study the nanomechanics of the cell nucleus. Using a custom-designed micro-magnetic-tweezers instrument, we can achieve sufficiently high magnetic forces enabling the application and measurement of controlled distortion of the internal nuclear structure on the nanometer scale. We precisely measure the elasticity and viscosity inside the nucleus of living HeLa cells. The high value of the Young's modulus (Y = 2.5 x 10(2) Pa) measured relative to the cytoplasm is explained by a large-scale model for in vivo chromatin structure using a polymer network model.",

keywords = "Chromatin, HeLa Cells, Humans, Nanotechnology, Journal Article, Research Support, Non-U.S. Gov't",

author = "{de Vries}, {Anthony H B} and Krenn, {Bea E} and {van Driel}, Roel and Vinod Subramaniam and Kanger, {Johannes S}",

year = "2007",

month = may,

doi = "10.1021/nl070603+",

language = "English",

volume = "7",

pages = "1424--7",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "5",

}

TY - JOUR

T1 - Direct observation of nanomechanical properties of chromatin in living cells

AU - de Vries, Anthony H B

AU - Krenn, Bea E

AU - van Driel, Roel

AU - Subramaniam, Vinod

AU - Kanger, Johannes S

PY - 2007/5

Y1 - 2007/5

N2 - Precise manipulation of nanometer-sized magnetic particles using magnetic tweezers has yielded insights into the rheology of the cell cytoplasm. We present first results using this approach to study the nanomechanics of the cell nucleus. Using a custom-designed micro-magnetic-tweezers instrument, we can achieve sufficiently high magnetic forces enabling the application and measurement of controlled distortion of the internal nuclear structure on the nanometer scale. We precisely measure the elasticity and viscosity inside the nucleus of living HeLa cells. The high value of the Young's modulus (Y = 2.5 x 10(2) Pa) measured relative to the cytoplasm is explained by a large-scale model for in vivo chromatin structure using a polymer network model.

AB - Precise manipulation of nanometer-sized magnetic particles using magnetic tweezers has yielded insights into the rheology of the cell cytoplasm. We present first results using this approach to study the nanomechanics of the cell nucleus. Using a custom-designed micro-magnetic-tweezers instrument, we can achieve sufficiently high magnetic forces enabling the application and measurement of controlled distortion of the internal nuclear structure on the nanometer scale. We precisely measure the elasticity and viscosity inside the nucleus of living HeLa cells. The high value of the Young's modulus (Y = 2.5 x 10(2) Pa) measured relative to the cytoplasm is explained by a large-scale model for in vivo chromatin structure using a polymer network model.

KW - Chromatin

KW - HeLa Cells

KW - Humans

KW - Nanotechnology

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1021/nl070603+

DO - 10.1021/nl070603+

M3 - Article

C2 - 17451276

SN - 1530-6984

VL - 7

SP - 1424

EP - 1427

JO - Nano Letters

JF - Nano Letters

IS - 5

ER -

Direct observation of nanomechanical properties of chromatin in living cells

Abstract

Keywords

Access to Document

Persistent URL (handle)

Fingerprint

Cite this