Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces

Maryana Escalante; Pascale Maury; Christiaan M Bruinink; Kees van der Werf; John D Olsen; John A Timney; Jurriaan Huskens; C Neil Hunter; Vinod Subramaniam; Cees Otto

doi:10.1088/0957-4484/19/02/025101

Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces

Maryana Escalante, Pascale Maury, Christiaan M Bruinink, Kees van der Werf, John D Olsen, John A Timney, Jurriaan Huskens, C Neil Hunter, Vinod Subramaniam, Cees Otto

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

We report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly(ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino-terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.

Original language	English
Pages (from-to)	025101
Journal	Nanotechnology
Volume	19
Issue number	2
DOIs	https://doi.org/10.1088/0957-4484/19/02/025101
Publication status	Published - 16 Jan 2008

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Journal Article

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title = "Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces",

abstract = "We report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly(ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino-terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.",

keywords = "Journal Article",

author = "Maryana Escalante and Pascale Maury and Bruinink, {Christiaan M} and {van der Werf}, Kees and Olsen, {John D} and Timney, {John A} and Jurriaan Huskens and {Neil Hunter}, C and Vinod Subramaniam and Cees Otto",

year = "2008",

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doi = "10.1088/0957-4484/19/02/025101",

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T1 - Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces

AU - Escalante, Maryana

AU - Maury, Pascale

AU - Bruinink, Christiaan M

AU - van der Werf, Kees

AU - Olsen, John D

AU - Timney, John A

AU - Huskens, Jurriaan

AU - Neil Hunter, C

AU - Subramaniam, Vinod

AU - Otto, Cees

PY - 2008/1/16

Y1 - 2008/1/16

N2 - We report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly(ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino-terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.

AB - We report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly(ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino-terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.

KW - Journal Article

U2 - 10.1088/0957-4484/19/02/025101

DO - 10.1088/0957-4484/19/02/025101

M3 - Article

C2 - 21817532

VL - 19

SP - 025101

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 2

ER -

Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces

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