Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader

B Schwer; P. Visca; J C Vos; H.G. Stunnenberg

doi:10.1016/0092-8674(87)90212-1

Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader

B Schwer, P. Visca, J C Vos, H.G. Stunnenberg

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the "start site" determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5' terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position -1, confirms the existence of a poly(A) leader of approximately 35 A-residues.

Original language	English
Pages (from-to)	163-9
Number of pages	7
Journal	Cell
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(87)90212-1
Publication status	Published - 17 Jul 1987

Keywords

Base Sequence
Cell Line
HeLa Cells
Humans
Poly A
RNA Processing, Post-Transcriptional
RNA, Messenger
Transcription, Genetic
Vaccinia virus
Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0092-8674(87)90212-1

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title = "Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader",

abstract = "We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the {"}start site{"} determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5' terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position -1, confirms the existence of a poly(A) leader of approximately 35 A-residues.",

keywords = "Base Sequence, Cell Line, HeLa Cells, Humans, Poly A, RNA Processing, Post-Transcriptional, RNA, Messenger, Transcription, Genetic, Vaccinia virus, Journal Article",

author = "B Schwer and P. Visca and Vos, {J C} and H.G. Stunnenberg",

year = "1987",

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doi = "10.1016/0092-8674(87)90212-1",

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T1 - Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader

AU - Schwer, B

AU - Visca, P.

AU - Vos, J C

AU - Stunnenberg, H.G.

PY - 1987/7/17

Y1 - 1987/7/17

N2 - We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the "start site" determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5' terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position -1, confirms the existence of a poly(A) leader of approximately 35 A-residues.

AB - We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the "start site" determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5' terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position -1, confirms the existence of a poly(A) leader of approximately 35 A-residues.

KW - Base Sequence

KW - Cell Line

KW - HeLa Cells

KW - Humans

KW - Poly A

KW - RNA Processing, Post-Transcriptional

KW - RNA, Messenger

KW - Transcription, Genetic

KW - Vaccinia virus

KW - Journal Article

U2 - 10.1016/0092-8674(87)90212-1

DO - 10.1016/0092-8674(87)90212-1

M3 - Article

C2 - 3594569

SN - 0092-8674

VL - 50

SP - 163

EP - 169

JO - Cell

JF - Cell

IS - 2

ER -

Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader

Abstract

Keywords

UN SDGs

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