Disruption of oxidative phosphorylation (OXPHOS) by hydroxylated polybrominated diphenyl ethers (OH-PBDEs) present in the marine environment

Jessica Legradi, Anna-Karin Dahlberg, Peter Cenijn, Göran Marsh, Lillemor Asplund, Åke Bergman, Juliette Legler

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are of growing concern, as they have been detected in both humans and wildlife and have been shown to be toxic. Recent studies have indicated that OH-PBDEs can be more toxic than PBDEs, partly due to their ability to disrupt oxidative phosphorylation (OXPHOS), an essential process in energy metabolism. In this study, we determined the OXPHOS disruption potential of 18 OH-PBDE congeners reported in marine wildlife using two in vitro bioassays, namely the classic rat mitochondrial respiration assay, and a mitochondrial membrane potential assay using zebrafish PAC2 cells. Single OH-PBDE congeners as well as mixtures were tested to study potential additive or synergistic effects. An environmental mixture composed of seven OH-PBDE congeners mimicking the concentrations reported in Baltic blue mussels were also studied. We report that all OH-PBDEs tested were able to disrupt OXPHOS via either protonophoric uncoupling and/or inhibition of the electron transport chain. Additionally we show that OH-PBDEs tested in combinations as found in the environment have the potential to disrupt OXPHOS. Importantly, mixtures of OH-PBDEs may show very strong synergistic effects, stressing the importance of further research on the in vivo impacts of these compounds in the environment.

Original languageEnglish
Pages (from-to)14703-11
Number of pages9
JournalEnvironmental Science and Technology
Volume48
Issue number24
DOIs
Publication statusPublished - 16 Dec 2014

Fingerprint

Halogenated Diphenyl Ethers
Oxidative Phosphorylation
Poisons
Mytilus edulis
Mitochondrial Membrane Potential
Zebrafish
Electron Transport
Biological Assay
Energy Metabolism
Respiration

Keywords

  • Adenosine Triphosphate/metabolism
  • Animals
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Ecotoxicology/methods
  • Embryo, Nonmammalian/cytology
  • Halogenated Diphenyl Ethers/toxicity
  • Humans
  • Hydroxylation
  • Membrane Potential, Mitochondrial/drug effects
  • Mytilus edulis/metabolism
  • Oxidative Phosphorylation/drug effects
  • Rats
  • Water Pollutants, Chemical/toxicity
  • Zebrafish/embryology

Cite this

Legradi, Jessica ; Dahlberg, Anna-Karin ; Cenijn, Peter ; Marsh, Göran ; Asplund, Lillemor ; Bergman, Åke ; Legler, Juliette. / Disruption of oxidative phosphorylation (OXPHOS) by hydroxylated polybrominated diphenyl ethers (OH-PBDEs) present in the marine environment. In: Environmental Science and Technology. 2014 ; Vol. 48, No. 24. pp. 14703-11.
@article{a95da2c97b7d4824a324d3113ddd967c,
title = "Disruption of oxidative phosphorylation (OXPHOS) by hydroxylated polybrominated diphenyl ethers (OH-PBDEs) present in the marine environment",
abstract = "Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are of growing concern, as they have been detected in both humans and wildlife and have been shown to be toxic. Recent studies have indicated that OH-PBDEs can be more toxic than PBDEs, partly due to their ability to disrupt oxidative phosphorylation (OXPHOS), an essential process in energy metabolism. In this study, we determined the OXPHOS disruption potential of 18 OH-PBDE congeners reported in marine wildlife using two in vitro bioassays, namely the classic rat mitochondrial respiration assay, and a mitochondrial membrane potential assay using zebrafish PAC2 cells. Single OH-PBDE congeners as well as mixtures were tested to study potential additive or synergistic effects. An environmental mixture composed of seven OH-PBDE congeners mimicking the concentrations reported in Baltic blue mussels were also studied. We report that all OH-PBDEs tested were able to disrupt OXPHOS via either protonophoric uncoupling and/or inhibition of the electron transport chain. Additionally we show that OH-PBDEs tested in combinations as found in the environment have the potential to disrupt OXPHOS. Importantly, mixtures of OH-PBDEs may show very strong synergistic effects, stressing the importance of further research on the in vivo impacts of these compounds in the environment.",
keywords = "Adenosine Triphosphate/metabolism, Animals, Cells, Cultured, Dose-Response Relationship, Drug, Ecotoxicology/methods, Embryo, Nonmammalian/cytology, Halogenated Diphenyl Ethers/toxicity, Humans, Hydroxylation, Membrane Potential, Mitochondrial/drug effects, Mytilus edulis/metabolism, Oxidative Phosphorylation/drug effects, Rats, Water Pollutants, Chemical/toxicity, Zebrafish/embryology",
author = "Jessica Legradi and Anna-Karin Dahlberg and Peter Cenijn and G{\"o}ran Marsh and Lillemor Asplund and {\AA}ke Bergman and Juliette Legler",
year = "2014",
month = "12",
day = "16",
doi = "10.1021/es5039744",
language = "English",
volume = "48",
pages = "14703--11",
journal = "Environmental Science and Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "24",

}

Disruption of oxidative phosphorylation (OXPHOS) by hydroxylated polybrominated diphenyl ethers (OH-PBDEs) present in the marine environment. / Legradi, Jessica; Dahlberg, Anna-Karin; Cenijn, Peter; Marsh, Göran; Asplund, Lillemor; Bergman, Åke; Legler, Juliette.

In: Environmental Science and Technology, Vol. 48, No. 24, 16.12.2014, p. 14703-11.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Disruption of oxidative phosphorylation (OXPHOS) by hydroxylated polybrominated diphenyl ethers (OH-PBDEs) present in the marine environment

AU - Legradi, Jessica

AU - Dahlberg, Anna-Karin

AU - Cenijn, Peter

AU - Marsh, Göran

AU - Asplund, Lillemor

AU - Bergman, Åke

AU - Legler, Juliette

PY - 2014/12/16

Y1 - 2014/12/16

N2 - Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are of growing concern, as they have been detected in both humans and wildlife and have been shown to be toxic. Recent studies have indicated that OH-PBDEs can be more toxic than PBDEs, partly due to their ability to disrupt oxidative phosphorylation (OXPHOS), an essential process in energy metabolism. In this study, we determined the OXPHOS disruption potential of 18 OH-PBDE congeners reported in marine wildlife using two in vitro bioassays, namely the classic rat mitochondrial respiration assay, and a mitochondrial membrane potential assay using zebrafish PAC2 cells. Single OH-PBDE congeners as well as mixtures were tested to study potential additive or synergistic effects. An environmental mixture composed of seven OH-PBDE congeners mimicking the concentrations reported in Baltic blue mussels were also studied. We report that all OH-PBDEs tested were able to disrupt OXPHOS via either protonophoric uncoupling and/or inhibition of the electron transport chain. Additionally we show that OH-PBDEs tested in combinations as found in the environment have the potential to disrupt OXPHOS. Importantly, mixtures of OH-PBDEs may show very strong synergistic effects, stressing the importance of further research on the in vivo impacts of these compounds in the environment.

AB - Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are of growing concern, as they have been detected in both humans and wildlife and have been shown to be toxic. Recent studies have indicated that OH-PBDEs can be more toxic than PBDEs, partly due to their ability to disrupt oxidative phosphorylation (OXPHOS), an essential process in energy metabolism. In this study, we determined the OXPHOS disruption potential of 18 OH-PBDE congeners reported in marine wildlife using two in vitro bioassays, namely the classic rat mitochondrial respiration assay, and a mitochondrial membrane potential assay using zebrafish PAC2 cells. Single OH-PBDE congeners as well as mixtures were tested to study potential additive or synergistic effects. An environmental mixture composed of seven OH-PBDE congeners mimicking the concentrations reported in Baltic blue mussels were also studied. We report that all OH-PBDEs tested were able to disrupt OXPHOS via either protonophoric uncoupling and/or inhibition of the electron transport chain. Additionally we show that OH-PBDEs tested in combinations as found in the environment have the potential to disrupt OXPHOS. Importantly, mixtures of OH-PBDEs may show very strong synergistic effects, stressing the importance of further research on the in vivo impacts of these compounds in the environment.

KW - Adenosine Triphosphate/metabolism

KW - Animals

KW - Cells, Cultured

KW - Dose-Response Relationship, Drug

KW - Ecotoxicology/methods

KW - Embryo, Nonmammalian/cytology

KW - Halogenated Diphenyl Ethers/toxicity

KW - Humans

KW - Hydroxylation

KW - Membrane Potential, Mitochondrial/drug effects

KW - Mytilus edulis/metabolism

KW - Oxidative Phosphorylation/drug effects

KW - Rats

KW - Water Pollutants, Chemical/toxicity

KW - Zebrafish/embryology

U2 - 10.1021/es5039744

DO - 10.1021/es5039744

M3 - Article

VL - 48

SP - 14703

EP - 14711

JO - Environmental Science and Technology

JF - Environmental Science and Technology

SN - 0013-936X

IS - 24

ER -