Dissection of rodent brain regions: Guided free-hand slicing and dissection of frozen tissue

Sabine Spijker*, Leonidas Faliagkas, Priyanka Rao-Ruiz

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Previously we reported on a free-hand dissection guide for proteomics samples, where the major focus was on dissection of fresh brain tissue. Although the fresh brain provides clear advantages, such as being able to see structures in very great detail by differences in color and shade, it also has some disadvantages. This particularly becomes evident when processing multiple samples within a short time-window, e.g., after a physiological stimulus. In this chapter, we discuss some of the current methods to take tissue samples from frozen tissue, keeping in mind the requirements for subcellular isolation (e.g., synaptosomes) of proteomics samples. In addition, we provide a step-by-step protocol to improve standardization of tissue dissection in your lab.

Original languageEnglish
Title of host publicationNeuroproteomics
EditorsKa Wan Li
PublisherHumana Press Inc.
Pages7-19
Number of pages13
Edition2nd
ISBN (Electronic)9781493996629
ISBN (Print)9781493996612
DOIs
Publication statusPublished - 2019

Publication series

NameNeuromethods
Volume146
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Brain
  • Frozen tissue
  • Guided free-hand dissection
  • Mouse
  • Rat

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