Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

Y.C. Lin; C.P. Broedersz; A.C. Rowat; T. Wedig; H. Herrmann; F.C. MacKintosh; D. A. Weitz

doi:10.1016/j.jmb.2010.04.054

Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

Y.C. Lin, C.P. Broedersz, A.C. Rowat, T. Wedig, H. Herrmann, F.C. MacKintosh, D. A. Weitz

LaserLaB - Molecular Biophysics

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Intermediate filament networks in the cytoplasm and nucleus are critical for the mechanical integrity of metazoan cells. However, the mechanism of crosslinking in these networks and the origins of their mechanical properties are not understood. Here, we study the elastic behavior of in vitro networks of the intermediate filament protein vimentin. Rheological experiments reveal that vimentin networks stiffen with increasing concentrations of Ca

Original language	English
Pages (from-to)	637-644
Number of pages	8
Journal	Journal of Molecular Biology
Volume	399
Issue number	4
DOIs	https://doi.org/10.1016/j.jmb.2010.04.054
Publication status	Published - 2010

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10.1016/j.jmb.2010.04.054

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title = "Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics",

abstract = "Intermediate filament networks in the cytoplasm and nucleus are critical for the mechanical integrity of metazoan cells. However, the mechanism of crosslinking in these networks and the origins of their mechanical properties are not understood. Here, we study the elastic behavior of in vitro networks of the intermediate filament protein vimentin. Rheological experiments reveal that vimentin networks stiffen with increasing concentrations of Ca",

author = "Y.C. Lin and C.P. Broedersz and A.C. Rowat and T. Wedig and H. Herrmann and F.C. MacKintosh and Weitz, {D. A.}",

year = "2010",

doi = "10.1016/j.jmb.2010.04.054",

language = "English",

volume = "399",

pages = "637--644",

journal = "Journal of Molecular Biology",

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T1 - Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

AU - Lin, Y.C.

AU - Broedersz, C.P.

AU - Rowat, A.C.

AU - Wedig, T.

AU - Herrmann, H.

AU - MacKintosh, F.C.

AU - Weitz, D. A.

PY - 2010

Y1 - 2010

N2 - Intermediate filament networks in the cytoplasm and nucleus are critical for the mechanical integrity of metazoan cells. However, the mechanism of crosslinking in these networks and the origins of their mechanical properties are not understood. Here, we study the elastic behavior of in vitro networks of the intermediate filament protein vimentin. Rheological experiments reveal that vimentin networks stiffen with increasing concentrations of Ca

AB - Intermediate filament networks in the cytoplasm and nucleus are critical for the mechanical integrity of metazoan cells. However, the mechanism of crosslinking in these networks and the origins of their mechanical properties are not understood. Here, we study the elastic behavior of in vitro networks of the intermediate filament protein vimentin. Rheological experiments reveal that vimentin networks stiffen with increasing concentrations of Ca

U2 - 10.1016/j.jmb.2010.04.054

DO - 10.1016/j.jmb.2010.04.054

M3 - Article

SN - 0022-2836

VL - 399

SP - 637

EP - 644

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

Abstract

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