DNA Damage, n-3 Long-Chain PUFA Levels and Proteomic Profile in Brazilian Children and Adolescents

T.T. Barros, V.D.P. Venâncio, L.C. Hernandes, Lusania Maria Greggi Antunes, E. Hillesheim, R.G. Salomão, M.G. Mathias, C.A. Coelho-Landell, R.B.D. Toffano, M.O.R. Do Vale Almada, J.S. Camelo-Junior, Sofia Moco, O. Cominetti, F.D.V. Ued, J. Kaput, Jacqueline Pontes Monteiro

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Fatty acids play a significant role in maintaining cellular and DNA protection and we previously found an inverse relationship between blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DNA damage. The aim of this study was to explore differences in proteomic profiles, for 117 pro-inflammatory proteins, in two previously defined groups of individuals with different DNA damage and EPA and DHA levels. Healthy children and adolescents (n = 140) aged 9 to 13 years old in an urban area of Brazil were divided by k-means cluster test into two clusters of DNA damage (tail intensity) using the comet assay (cluster 1 = 5.9% ± 1.2 and cluster 2 = 13.8% ± 3.1) in our previous study. The cluster with higher DNA damage and lower levels of DHA (6.2 ± 1.6 mg/dL; 5.4 ± 1.3 mg/dL, p = 0.003) and EPA (0.6 ± 0.2 mg/dL; 0.5 ± 0.1 mg/dL, p < 0.001) presented increased expression of the proteins CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB, which are involved in pro-inflammatory pathways. Our findings support the hypothesis that low levels of n-3 long-chain PUFA may have a less protective role against DNA damage through expression of pro-inflammatory proteins, such as CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB.
Original languageEnglish
Article number2483
Pages (from-to)1-12
Number of pages12
JournalNutrients
Volume13
Issue number8
Early online date21 Jul 2021
DOIs
Publication statusPublished - Aug 2021

Funding

Acknowledgments: We acknowledge all subjects and their families for participation. This study was supported by Nestlé Institute of Health Sciences and the São Paulo Research Foundation (FAPESP-protocol 2012/20421-8). T.T.B. was sponsored by a fellowship from National Council for Scientific and Technological Development (CNPq-protocol 131224/2015-8). This research was funded by Nestl? Institute of Health Sciences (NIHS), Lausanne, Switzer-land, contract reference RDHS 000054, and by Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP), S?o Paulo, Brazil, grant number 2012/20421-8. T.T.B. was sponsored by a fellowship from Conselho Nacional de Desenvolvimento Cient?fico e T?cnol?gico (CNPQ), Bras?lia, Brazil, grant number 131224/2015-8. We acknowledge all subjects and their families for participation. This study was supported by Nestl? Institute of Health Sciences and the S?o Paulo Research Foundation (FAPESP-protocol 2012/20421-8). T.T.B. was sponsored by a fellowship from National Council for Scientific and Technological Development (CNPq-protocol 131224/2015-8). Funding: This research was funded by Nestlé Institute of Health Sciences (NIHS), Lausanne, Switzerland, contract reference RDHS 000054, and by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), São Paulo, Brazil, grant number 2012/20421-8. T.T.B. was sponsored by a fellowship from Conselho Nacional de Desenvolvimento Científico e Técnológico (CNPQ), Brasília, Brazil, grant number 131224/2015-8.

FundersFunder number
CNPq-protocol
Conselho Nacional de Desenvolvimento Cient?fico e T?cnol?gico
Conselho Nacional de Desenvolvimento Científico e Técnológico
FAPESP-protocol
Institute of Health Sciences
NIHS
Nestlé Institute of Health SciencesRDHS 000054
Fundação de Amparo à Pesquisa do Estado de São Paulo2012/20421-8
Conselho Nacional de Desenvolvimento Científico e Tecnológico131224/2015-8

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