Domain exchange at the 3' end of the gene encoding the fratricide meningococcal two-partner secretion protein A

J. Arenas, K. Schipper, P. van Ulsen, A. van den Ende, J. Tommassen

Research output: Contribution to JournalArticleAcademicpeer-review


Background: Two-partner secretion systems in Gram-negative bacteria consist of an outer membrane protein TpsB that mediates the secretion of a cognate TpsA protein into the extracellular milieu. TpsA proteins have diverse, often virulence-related functions, and some of them inhibit the growth of related bacteria. In Neisseria meningitidis, several functions have been attributed to the TpsA proteins. Downstream of the tpsB and tpsA genes, several shorter tpsA-related gene cassettes, called tpsC, are located interspersed with intervening open-reading frames (IORFs). It has been suggested that the tpsC cassettes may recombine with the tpsA gene as a mechanism of antigenic variation. Here, we investigated (i) whether TpsA of N. meningitidis also has growth-inhibitory properties, (ii) whether tpsC cassettes recombine with the tpsA gene, and (iii) what the consequences of such recombination events might be.Results: We demonstrate that meningococcal TpsA has growth-inhibitory properties and that the IORF located immediately downstream of tpsA confers immunity to the producing strain. Although bioinformatics analysis suggests that recombination between tpsC cassettes and tpsA occurs, detailed analysis of the tpsA gene in a large collection of disease isolates of three clonal complexes revealed that the frequency is very low and cannot be a mechanism of antigenic variation. However, recombination affected growth inhibition. In vitro experiments revealed that recombination can be mediated through acquirement of tpsC cassettes from the environment and it identified the regions involved in the recombination.Conclusions: Meningococcal TpsA has growth-inhibitory properties. Recombination between tpsA and tpsC cassettes occurs in vivo but is rare and has consequences for growth inhibition. A recombination model is proposed and we propose that the main goal of recombination is the collection of new IORFs for protection against a variety of TpsA proteins. © 2013 Arenas et al.; licensee BioMed Central Ltd.
Original languageEnglish
Article number622
JournalBMC Genomics
Publication statusPublished - 2013

Bibliographical note

1471-2164 Arenas, Jesus Schipper, Kim van Ulsen, Peter van der Ende, Arie Tommassen, Jan Journal Article Research Support, Non-U.S. Gov't England BMC Genomics. 2013 Sep 14;14:622. doi: 10.1186/1471-2164-14-622.


Dive into the research topics of 'Domain exchange at the 3' end of the gene encoding the fratricide meningococcal two-partner secretion protein A'. Together they form a unique fingerprint.

Cite this