Dominance of Geobacteraceae in BTX-grading enrichments from an iron-reducing aquifer.

S Botton, M. van Harmelen, M. Braster, J. Parsons, W.F.M. Roling

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Microbial community structure was linked to degradation potential in benzene-, toluene- or xylene- (BTX) degrading, iron-reducing enrichments derived from an iron-reducing aquifer polluted with landfill leachate. Enrichments were characterized using 16S rRNA gene-based analysis, targeting of the benzylsuccinate synthase-encoding bssA gene and phospholipid fatty acid (PLFA) profiling in combination with tracking of labelled substrate. 16S rRNA gene analysis indicated the dominance of Geobacteraceae, and one phylotype in particular, in all enrichments inoculated with polluted aquifer material. Upon cultivation, progressively higher degradation rates with a concomitant decrease in species richness occurred in all primary incubations and successive enrichments. Yet, the same Geobacteraceae phylotype remained common and dominant, indicating its involvement in BTX degradation. However, the bssA gene sequences in BTX degrading enrichments differed considerably from those of Geobacter isolates, suggesting that the first steps of toluene, but also benzene and xylene oxidation, are carried out by another member of the enrichments. Therefore, BTX would be synthrophically degraded by a bacterial consortium in which Geobacteraceae utilized intermediate metabolites. PLFA analysis in combination with
Original languageEnglish
Pages (from-to)118-130
Number of pages13
JournalFEMS Microbiology Ecology
Volume62
DOIs
Publication statusPublished - 2007

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Xylenes
Groundwater
Toluene
xylene
Benzene
toluene
benzene
Iron
aquifer
iron
rRNA Genes
gene
phospholipid
Phospholipids
Fatty Acids
Geobacter
Chemical Water Pollutants
degradation
fatty acid
Genes

Cite this

Botton, S ; van Harmelen, M. ; Braster, M. ; Parsons, J. ; Roling, W.F.M. / Dominance of Geobacteraceae in BTX-grading enrichments from an iron-reducing aquifer. In: FEMS Microbiology Ecology. 2007 ; Vol. 62. pp. 118-130.
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abstract = "Microbial community structure was linked to degradation potential in benzene-, toluene- or xylene- (BTX) degrading, iron-reducing enrichments derived from an iron-reducing aquifer polluted with landfill leachate. Enrichments were characterized using 16S rRNA gene-based analysis, targeting of the benzylsuccinate synthase-encoding bssA gene and phospholipid fatty acid (PLFA) profiling in combination with tracking of labelled substrate. 16S rRNA gene analysis indicated the dominance of Geobacteraceae, and one phylotype in particular, in all enrichments inoculated with polluted aquifer material. Upon cultivation, progressively higher degradation rates with a concomitant decrease in species richness occurred in all primary incubations and successive enrichments. Yet, the same Geobacteraceae phylotype remained common and dominant, indicating its involvement in BTX degradation. However, the bssA gene sequences in BTX degrading enrichments differed considerably from those of Geobacter isolates, suggesting that the first steps of toluene, but also benzene and xylene oxidation, are carried out by another member of the enrichments. Therefore, BTX would be synthrophically degraded by a bacterial consortium in which Geobacteraceae utilized intermediate metabolites. PLFA analysis in combination with",
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Dominance of Geobacteraceae in BTX-grading enrichments from an iron-reducing aquifer. / Botton, S; van Harmelen, M.; Braster, M.; Parsons, J.; Roling, W.F.M.

In: FEMS Microbiology Ecology, Vol. 62, 2007, p. 118-130.

Research output: Contribution to JournalArticleAcademicpeer-review

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