Dopant-enriched nitrogen gas to boost ionization of glycoproteins analyzed with native liquid chromatography coupled to nano-electrospray ionization

Funding

This work was supported by the Dutch Research Council (SATIN project, Grant No. 731.017.202 ) and the LUMC Fellowship 2020 to E.D.V. The authors thank DSM Science & Innovation (Biodata & Translation, Center for Analytical Innovation) for supplying the industrial enzymes. This work was supported by the Dutch Research Council (SATIN project, Grant No. 731.017.202) and the LUMC Fellowship 2020 to E.D.V. The authors thank DSM Science & Innovation (Biodata & Translation, Center for Analytical Innovation) for supplying the industrial enzymes.Supporting Information: AEX-UV chromatograms (Fig. S1); Mass spectra of RNase B with and without DEN gas (Fig. S2); Influence DEN gas on mAb ionization (Fig. S3) Effect of DEN gas on ionization of ovalbumin (Fig. S4); MS methods of the proteins (Table S1); MS Intensity of assigned proteoforms using the different DEN gases (Table S2) (PDF).