Dynamic changes between two LHCX-related energy quenching sites control diatom photoacclimation

Lucilla Taddei, Volha U. Chukhutsina, Bernard Lepetit, Giulio Rocco Stella, Roberto Bassi, Herbert van Amerongen, Jean Pierre Bouly, Marianne Jaubert, Giovanni Finazzi*, Angela Falciatore

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

10 Downloads (Pure)

Abstract

Marine diatoms are prominent phytoplankton organisms that perform photosynthesis in extremely variable environments. Diatoms possess a strong ability to dissipate excess absorbed energy as heat via nonphotochemical quenching (NPQ). This process relies on changes in carotenoid pigment composition (xanthophyll cycle) and on specific members of the light-harvesting complex family specialized in photoprotection (LHCXs), which potentially act as NPQ effectors. However, the link between light stress, NPQ, and the existence of different LHCX isoforms is not understood in these organisms. Using picosecond fluorescence analysis, we observed two types of NPQ in the pennate diatom Phaeodactylum tricornutum that were dependent on light conditions. Short exposure of low-light-acclimated cells to high light triggers the onset of energy quenching close to the core of photosystem II, while prolonged light stress activates NPQ in the antenna. Biochemical analysis indicated a link between the changes in the NPQ site/mechanism and the induction of different LHCX isoforms, which accumulate either in the antenna complexes or in the core complex. By comparing the responses of wild-type cells and transgenic lines with a reduced expression of the major LHCX isoform, LHCX1, we conclude that core complex-associated NPQ is more effective in photoprotection than is the antenna complex. Overall, our data clarify the complex molecular scenario of light responses in diatoms and provide a rationale for the existence of a degenerate family of LHCX proteins in these algae.

Original languageEnglish
Pages (from-to)953-965
Number of pages13
JournalPlant Physiology
Volume177
Issue number3
Early online date17 May 2018
DOIs
Publication statusPublished - Jul 2018

Funding

1This work was supported by the Marie Curie Initial Training Network Accliphot (FP7-PEPOPLE-2012-ITN; 316427) to A.F., G.F., and R.B., the Agence Nationale de la Recherche (ANR-12-BIME DiaDomOil) to A.F. and G.F., the Marie Curie Initial Training Network CALIPSO (ITN 2013 GA 607607) to A.F., the Human Frontier Science Program (HFSP0052) and the LabEx GRAL (ANR-10-LABX-49-01) to G.F., and the Zukunftskolleg Konstanz and the Deutsche Forschungsgemeinschaft (DFG-LE 3358/3-1) to B.L.

FundersFunder number
ANR-12-BIME DiaDomOilITN 2013 GA 607607
LabEx GRALANR-10-LABX-49-01
Zukunftskolleg Konstanz
Human Frontier Science ProgramHFSP0052
Deutsche ForschungsgemeinschaftDFG-LE 3358/3-1
Agence Nationale de la Recherche

    Fingerprint

    Dive into the research topics of 'Dynamic changes between two LHCX-related energy quenching sites control diatom photoacclimation'. Together they form a unique fingerprint.

    Cite this