Effect of Light Acclimation on the Organization of Photosystem II Super- and Sub-Complexes in Arabidopsis thaliana

L.W. Bielczynski, G. Schansker, R. Croce

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

To survive under highly variable environmental conditions, higher plants have acquired a large variety of acclimation responses. Different strategies are used to cope with changes in light intensity with the common goal of modulating the functional antenna size of Photosystem II (PSII). Here we use a combination of biochemical and biophysical methods to study these changes in response to acclimation to high light (HL). After 2 h of exposure, a decrease in the amount of the large PSII supercomplexes is observed indicating that plants are already acclimating to HL at this stage. It is also shown that in HL the relative amount of antenna proteins decreases but this decrease is far less than the observed decrease of the functional antenna size, suggesting that part of the antenna present in the membranes in HL does not transfer energy efficiently to the reaction center. Finally, we observed LHCII monomers in all conditions. As the solubilization conditions used do not lead to monomerization of purified LHCII trimers, we should conclude that a population of LHCII monomers exists in the membrane. The relative amount of LHCII monomers strongly increases in plants acclimated to HL, while no changes in the trimer to monomer ratio are observed upon short exposure to stress.
Original languageEnglish
Article number105
JournalFrontiers in Plant Science
Volume7
DOIs
Publication statusPublished - 2016

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photosystem II
acclimation
Arabidopsis thaliana
antennae
energy transfer
solubilization
light intensity
environmental factors
proteins
methodology

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title = "Effect of Light Acclimation on the Organization of Photosystem II Super- and Sub-Complexes in Arabidopsis thaliana",
abstract = "To survive under highly variable environmental conditions, higher plants have acquired a large variety of acclimation responses. Different strategies are used to cope with changes in light intensity with the common goal of modulating the functional antenna size of Photosystem II (PSII). Here we use a combination of biochemical and biophysical methods to study these changes in response to acclimation to high light (HL). After 2 h of exposure, a decrease in the amount of the large PSII supercomplexes is observed indicating that plants are already acclimating to HL at this stage. It is also shown that in HL the relative amount of antenna proteins decreases but this decrease is far less than the observed decrease of the functional antenna size, suggesting that part of the antenna present in the membranes in HL does not transfer energy efficiently to the reaction center. Finally, we observed LHCII monomers in all conditions. As the solubilization conditions used do not lead to monomerization of purified LHCII trimers, we should conclude that a population of LHCII monomers exists in the membrane. The relative amount of LHCII monomers strongly increases in plants acclimated to HL, while no changes in the trimer to monomer ratio are observed upon short exposure to stress.",
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language = "English",
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journal = "Frontiers in Plant Science",
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Effect of Light Acclimation on the Organization of Photosystem II Super- and Sub-Complexes in Arabidopsis thaliana. / Bielczynski, L.W.; Schansker, G.; Croce, R.

In: Frontiers in Plant Science, Vol. 7, 105, 2016.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of Light Acclimation on the Organization of Photosystem II Super- and Sub-Complexes in Arabidopsis thaliana

AU - Bielczynski, L.W.

AU - Schansker, G.

AU - Croce, R.

PY - 2016

Y1 - 2016

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AB - To survive under highly variable environmental conditions, higher plants have acquired a large variety of acclimation responses. Different strategies are used to cope with changes in light intensity with the common goal of modulating the functional antenna size of Photosystem II (PSII). Here we use a combination of biochemical and biophysical methods to study these changes in response to acclimation to high light (HL). After 2 h of exposure, a decrease in the amount of the large PSII supercomplexes is observed indicating that plants are already acclimating to HL at this stage. It is also shown that in HL the relative amount of antenna proteins decreases but this decrease is far less than the observed decrease of the functional antenna size, suggesting that part of the antenna present in the membranes in HL does not transfer energy efficiently to the reaction center. Finally, we observed LHCII monomers in all conditions. As the solubilization conditions used do not lead to monomerization of purified LHCII trimers, we should conclude that a population of LHCII monomers exists in the membrane. The relative amount of LHCII monomers strongly increases in plants acclimated to HL, while no changes in the trimer to monomer ratio are observed upon short exposure to stress.

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