Effect of Vanadium chloroperoxidase on Enterococcus faecalis biofilms

I.F. Persoon; M.A. Hoogenkamp; A. Bury; P.R. Wesselink; A.F. Hartog; R. Wever; W. Crielaard

doi:10.1016/j.joen.2011.09.003

Effect of Vanadium chloroperoxidase on Enterococcus faecalis biofilms

I.F. Persoon
, M.A. Hoogenkamp
, A. Bury
, P.R. Wesselink
, A.F. Hartog
, R. Wever
, W. Crielaard

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Introduction
The aim of this study was to explore the antimicrobial effect of vanadiumchloroperoxidase (VCPO) reaction products on Enterococcusfaecalisbiofilms of 4 different strains.

Methods
Twenty-four-hour biofilms of E. faecalis strains V583, ER5/1, E2, and OS-16 were incubated in mixtures with VCPO, halide (either bromide or chloride), and hydrogen peroxide. The antibacterial efficacy was assessed by colony-forming unit counts.

Results
The VCPO reaction products had a similar efficacy in reducing the viability of the 4 strains of E. faecalis (94%; range, 87%-100%). Bromide as the halogen of choice was more effective on E. faecalis strains E2 and OS-16, as compared with chloride (Mann-Whitney U test; P < .05). Despite different quantities of produced biofilms by the 4 strains, VCPO treatment was similarly effective toward all strains (Kruskal-Wallis test; P < .05).

Conclusions
VCPO treatment results in an antimicrobial effect toward in vitro E. faecalisbiofilms and might provide an addition to current endodontic treatment, possibly as an antimicrobial dressing.

Original language	English
Pages (from-to)	72-74
Journal	The Journal of Endodontics
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.joen.2011.09.003
Publication status	Published - 2012

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10.1016/j.joen.2011.09.003

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@article{f04e6e1f3c73459c884841b0e36eb31f,

title = "Effect of Vanadium chloroperoxidase on Enterococcus faecalis biofilms",

abstract = "Introduction The aim of this study was to explore the antimicrobial effect of vanadiumchloroperoxidase (VCPO) reaction products on Enterococcusfaecalisbiofilms of 4 different strains. Methods Twenty-four-hour biofilms of E. faecalis strains V583, ER5/1, E2, and OS-16 were incubated in mixtures with VCPO, halide (either bromide or chloride), and hydrogen peroxide. The antibacterial efficacy was assessed by colony-forming unit counts. Results The VCPO reaction products had a similar efficacy in reducing the viability of the 4 strains of E. faecalis (94\%; range, 87\%-100\%). Bromide as the halogen of choice was more effective on E. faecalis strains E2 and OS-16, as compared with chloride (Mann-Whitney U test; P < .05). Despite different quantities of produced biofilms by the 4 strains, VCPO treatment was similarly effective toward all strains (Kruskal-Wallis test; P < .05). Conclusions VCPO treatment results in an antimicrobial effect toward in vitro E. faecalisbiofilms and might provide an addition to current endodontic treatment, possibly as an antimicrobial dressing.",

author = "I.F. Persoon and M.A. Hoogenkamp and A. Bury and P.R. Wesselink and A.F. Hartog and R. Wever and W. Crielaard",

year = "2012",

doi = "10.1016/j.joen.2011.09.003",

language = "English",

volume = "38",

pages = "72--74",

journal = "The Journal of Endodontics",

issn = "0099-2399",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Effect of Vanadium chloroperoxidase on Enterococcus faecalis biofilms

AU - Persoon, I.F.

AU - Hoogenkamp, M.A.

AU - Bury, A.

AU - Wesselink, P.R.

AU - Hartog, A.F.

AU - Wever, R.

AU - Crielaard, W.

PY - 2012

Y1 - 2012

N2 - Introduction The aim of this study was to explore the antimicrobial effect of vanadiumchloroperoxidase (VCPO) reaction products on Enterococcusfaecalisbiofilms of 4 different strains. Methods Twenty-four-hour biofilms of E. faecalis strains V583, ER5/1, E2, and OS-16 were incubated in mixtures with VCPO, halide (either bromide or chloride), and hydrogen peroxide. The antibacterial efficacy was assessed by colony-forming unit counts. Results The VCPO reaction products had a similar efficacy in reducing the viability of the 4 strains of E. faecalis (94%; range, 87%-100%). Bromide as the halogen of choice was more effective on E. faecalis strains E2 and OS-16, as compared with chloride (Mann-Whitney U test; P < .05). Despite different quantities of produced biofilms by the 4 strains, VCPO treatment was similarly effective toward all strains (Kruskal-Wallis test; P < .05). Conclusions VCPO treatment results in an antimicrobial effect toward in vitro E. faecalisbiofilms and might provide an addition to current endodontic treatment, possibly as an antimicrobial dressing.

AB - Introduction The aim of this study was to explore the antimicrobial effect of vanadiumchloroperoxidase (VCPO) reaction products on Enterococcusfaecalisbiofilms of 4 different strains. Methods Twenty-four-hour biofilms of E. faecalis strains V583, ER5/1, E2, and OS-16 were incubated in mixtures with VCPO, halide (either bromide or chloride), and hydrogen peroxide. The antibacterial efficacy was assessed by colony-forming unit counts. Results The VCPO reaction products had a similar efficacy in reducing the viability of the 4 strains of E. faecalis (94%; range, 87%-100%). Bromide as the halogen of choice was more effective on E. faecalis strains E2 and OS-16, as compared with chloride (Mann-Whitney U test; P < .05). Despite different quantities of produced biofilms by the 4 strains, VCPO treatment was similarly effective toward all strains (Kruskal-Wallis test; P < .05). Conclusions VCPO treatment results in an antimicrobial effect toward in vitro E. faecalisbiofilms and might provide an addition to current endodontic treatment, possibly as an antimicrobial dressing.

U2 - 10.1016/j.joen.2011.09.003

DO - 10.1016/j.joen.2011.09.003

M3 - Article

SN - 0099-2399

VL - 38

SP - 72

EP - 74

JO - The Journal of Endodontics

JF - The Journal of Endodontics

IS - 1

ER -

Effect of Vanadium chloroperoxidase on Enterococcus faecalis biofilms

Abstract

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