Effects of antimony on enzymatic and non-enzymatic antioxidants in a metallicolous and a non-metallicolous population of Salvia spinosa L

Shakiba Rajabpoor, Seyed Majid Ghaderian*, Henk Schat

*Corresponding author for this work

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    The present study aimed at elucidating the role of antioxidants and stress metabolites in antimony (Sb) tolerance in a metallicolous (M), Sb[V]-hypertolerant population, and a non-metallicolous (NM) population of Salvia spinosa, particularly with regard to the question of whether they could be involved in constitutive Sb tolerance or, specifically, in Sb[V] hypertolerance in the M population. Plants were exposed in hydroponics to 0, 8, 24, 74, 221 μM Sb (Ш or V). Superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) activities, and the concentrations of phenolics, flavonoids, and proline in leaves were measured after 20 d. As potential stress/tolerance markers, the concentrations of chlorophyll a and b, anthocyanins, and those of total soluble and reducing sugars were also measured. Chlorophyll a concentration reflected the difference, both in Sb[III] and Sb[V] tolerance, between N and NM, and the higher toxicity of Sb[III], compared to Sb[V]. APX and proline accumulation were more induced in M than in NM, and more by Sb[V] than by Sb[III], which is theoretically compatible with a role in Sb[V] hypertolerance. CAT was more induced in M than in NM, but more by Sb[III] than Sb[V], suggesting that is not functional in Sb[V] hypertolerance. The other enzymes and compounds did not exhibit significant Sb redox status*population interactions, suggesting that they don't play a role in, specifically, Sb[V] hypertolerance in M, but at most in the constitutive Sb[III] or Sb[V] tolerance of the species.

    Original languageEnglish
    Pages (from-to)386-394
    Number of pages9
    JournalPlant Physiology and Biochemistry
    Volume144
    Early online date9 Oct 2019
    DOIs
    Publication statusPublished - 1 Nov 2019

    Funding

    We would like to thank the Graduate School of University of Isfahan for providing research facilities for this study. We also acknowledge Plant Antioxidant Center of Excellence (PACE), University of Isfahan, Iran.

    FundersFunder number
    University of Isfahan
    Graduate School, Chongqing University

      Keywords

      • Antimony
      • Enzymatic antioxidants
      • Non-enzymatic antioxidants
      • Salvia spinosa

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