Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone

Joost Buskermolen, Karen Van Der Meijden, Regula Furrer, Dirk Jan Mons, Huib W. Van Essen, Annemieke C. Heijboer, Paul Lips, Richard T. Jaspers, Nathalie Bravenboer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Objectives. Mechanical loading may be an important factor in the regulation of bone derived hormones involved in phosphate homeostasis. This study investigated the effects of peak power and endurance training on expression levels of fibroblast growth factor 23 (FGF23) and 1α-hydroxylase (CYP27b1) in bone. Methods. Thirty-eight rats were assigned to six weeks of training in four groups: peak power (PT), endurance (ET), PT followed by ET (PET) or no training (control). In cortical bone, FGF23 was quantified using immunohistochemistry. mRNA expression levels of proteins involved in phosphate and vitamin D homeostasis were quantified in cortical bone and kidney. C-terminal FGF23, 25-hydroxyvitamin D3, parathyroid hormone (PTH), calcium and phosphate concentrations were measured in plasma or serum. Results. Neither FGF23 mRNA and protein expression levels in cortical bone nor FGF23 plasma concentrations differed between the groups. In cortical bone, mRNA expression levels of sclerostin (SOST), dental matrix protein 1 (DMP1), phosphate-regulating gene with homologies to endopeptidases on the X chromosome (PHEX) and matrix extracellular phosphoglycoprotein (MEPE) were lower after PT compared to ET and PET. Expression levels of CYP27b1 and vitamin D receptor (VDR) in tibial bone were decreased after PT compared to ET. In kidney, no differences between groups were observed for mRNA expression levels of CYP27b1, 24-hydroxylase (CYP24), VDR, NaPi-IIa cotransporter (NPT2a) and NaPi-IIc cotransporter (NPT2c). Serum PTH concentrations were higher after PT compared to controls. Conclusion. After six weeks, none of the training modalities induced changes in FGF23 expression levels. However, PT might have caused changes in local phosphate regulation within bone compared to ET and PET. CYP27b1 and VDR expression in bone was reduced after PT compared to ET, suggesting high intensity peak power training in this rat model is associated with decreased vitamin D signalling in bone.

Original languageEnglish
Article numbere6184
JournalPeerJ
Volume2019
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

vitamin D
Metabolism
Vitamin D
Rats
homeostasis
Bone
Homeostasis
fibroblast growth factors
Phosphates
bones
phosphates
Bone and Bones
Calcitriol Receptors
metabolism
rats
Messenger RNA
Mixed Function Oxygenases
Parathyroid Hormone
parathyroid hormone
Kidney

Keywords

  • 1α-hydroxylation
  • Bone
  • Exercise
  • FGF23
  • Mechanical loading
  • Phosphate homeostasis
  • Rat
  • Vitamin D

Cite this

Buskermolen, J., Van Der Meijden, K., Furrer, R., Mons, D. J., Van Essen, H. W., Heijboer, A. C., ... Bravenboer, N. (2019). Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone. PeerJ, 2019(1), [e6184]. https://doi.org/10.7717/peerj.6184
Buskermolen, Joost ; Van Der Meijden, Karen ; Furrer, Regula ; Mons, Dirk Jan ; Van Essen, Huib W. ; Heijboer, Annemieke C. ; Lips, Paul ; Jaspers, Richard T. ; Bravenboer, Nathalie. / Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone. In: PeerJ. 2019 ; Vol. 2019, No. 1.
@article{a7851882560640f1bd216ab71c1d88b5,
title = "Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone",
abstract = "Objectives. Mechanical loading may be an important factor in the regulation of bone derived hormones involved in phosphate homeostasis. This study investigated the effects of peak power and endurance training on expression levels of fibroblast growth factor 23 (FGF23) and 1α-hydroxylase (CYP27b1) in bone. Methods. Thirty-eight rats were assigned to six weeks of training in four groups: peak power (PT), endurance (ET), PT followed by ET (PET) or no training (control). In cortical bone, FGF23 was quantified using immunohistochemistry. mRNA expression levels of proteins involved in phosphate and vitamin D homeostasis were quantified in cortical bone and kidney. C-terminal FGF23, 25-hydroxyvitamin D3, parathyroid hormone (PTH), calcium and phosphate concentrations were measured in plasma or serum. Results. Neither FGF23 mRNA and protein expression levels in cortical bone nor FGF23 plasma concentrations differed between the groups. In cortical bone, mRNA expression levels of sclerostin (SOST), dental matrix protein 1 (DMP1), phosphate-regulating gene with homologies to endopeptidases on the X chromosome (PHEX) and matrix extracellular phosphoglycoprotein (MEPE) were lower after PT compared to ET and PET. Expression levels of CYP27b1 and vitamin D receptor (VDR) in tibial bone were decreased after PT compared to ET. In kidney, no differences between groups were observed for mRNA expression levels of CYP27b1, 24-hydroxylase (CYP24), VDR, NaPi-IIa cotransporter (NPT2a) and NaPi-IIc cotransporter (NPT2c). Serum PTH concentrations were higher after PT compared to controls. Conclusion. After six weeks, none of the training modalities induced changes in FGF23 expression levels. However, PT might have caused changes in local phosphate regulation within bone compared to ET and PET. CYP27b1 and VDR expression in bone was reduced after PT compared to ET, suggesting high intensity peak power training in this rat model is associated with decreased vitamin D signalling in bone.",
keywords = "1α-hydroxylation, Bone, Exercise, FGF23, Mechanical loading, Phosphate homeostasis, Rat, Vitamin D",
author = "Joost Buskermolen and {Van Der Meijden}, Karen and Regula Furrer and Mons, {Dirk Jan} and {Van Essen}, {Huib W.} and Heijboer, {Annemieke C.} and Paul Lips and Jaspers, {Richard T.} and Nathalie Bravenboer",
year = "2019",
month = "1",
day = "1",
doi = "10.7717/peerj.6184",
language = "English",
volume = "2019",
journal = "PeerJ",
issn = "2167-8359",
publisher = "PeerJ",
number = "1",

}

Buskermolen, J, Van Der Meijden, K, Furrer, R, Mons, DJ, Van Essen, HW, Heijboer, AC, Lips, P, Jaspers, RT & Bravenboer, N 2019, 'Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone' PeerJ, vol. 2019, no. 1, e6184. https://doi.org/10.7717/peerj.6184

Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone. / Buskermolen, Joost; Van Der Meijden, Karen; Furrer, Regula; Mons, Dirk Jan; Van Essen, Huib W.; Heijboer, Annemieke C.; Lips, Paul; Jaspers, Richard T.; Bravenboer, Nathalie.

In: PeerJ, Vol. 2019, No. 1, e6184, 01.01.2019.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone

AU - Buskermolen, Joost

AU - Van Der Meijden, Karen

AU - Furrer, Regula

AU - Mons, Dirk Jan

AU - Van Essen, Huib W.

AU - Heijboer, Annemieke C.

AU - Lips, Paul

AU - Jaspers, Richard T.

AU - Bravenboer, Nathalie

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objectives. Mechanical loading may be an important factor in the regulation of bone derived hormones involved in phosphate homeostasis. This study investigated the effects of peak power and endurance training on expression levels of fibroblast growth factor 23 (FGF23) and 1α-hydroxylase (CYP27b1) in bone. Methods. Thirty-eight rats were assigned to six weeks of training in four groups: peak power (PT), endurance (ET), PT followed by ET (PET) or no training (control). In cortical bone, FGF23 was quantified using immunohistochemistry. mRNA expression levels of proteins involved in phosphate and vitamin D homeostasis were quantified in cortical bone and kidney. C-terminal FGF23, 25-hydroxyvitamin D3, parathyroid hormone (PTH), calcium and phosphate concentrations were measured in plasma or serum. Results. Neither FGF23 mRNA and protein expression levels in cortical bone nor FGF23 plasma concentrations differed between the groups. In cortical bone, mRNA expression levels of sclerostin (SOST), dental matrix protein 1 (DMP1), phosphate-regulating gene with homologies to endopeptidases on the X chromosome (PHEX) and matrix extracellular phosphoglycoprotein (MEPE) were lower after PT compared to ET and PET. Expression levels of CYP27b1 and vitamin D receptor (VDR) in tibial bone were decreased after PT compared to ET. In kidney, no differences between groups were observed for mRNA expression levels of CYP27b1, 24-hydroxylase (CYP24), VDR, NaPi-IIa cotransporter (NPT2a) and NaPi-IIc cotransporter (NPT2c). Serum PTH concentrations were higher after PT compared to controls. Conclusion. After six weeks, none of the training modalities induced changes in FGF23 expression levels. However, PT might have caused changes in local phosphate regulation within bone compared to ET and PET. CYP27b1 and VDR expression in bone was reduced after PT compared to ET, suggesting high intensity peak power training in this rat model is associated with decreased vitamin D signalling in bone.

AB - Objectives. Mechanical loading may be an important factor in the regulation of bone derived hormones involved in phosphate homeostasis. This study investigated the effects of peak power and endurance training on expression levels of fibroblast growth factor 23 (FGF23) and 1α-hydroxylase (CYP27b1) in bone. Methods. Thirty-eight rats were assigned to six weeks of training in four groups: peak power (PT), endurance (ET), PT followed by ET (PET) or no training (control). In cortical bone, FGF23 was quantified using immunohistochemistry. mRNA expression levels of proteins involved in phosphate and vitamin D homeostasis were quantified in cortical bone and kidney. C-terminal FGF23, 25-hydroxyvitamin D3, parathyroid hormone (PTH), calcium and phosphate concentrations were measured in plasma or serum. Results. Neither FGF23 mRNA and protein expression levels in cortical bone nor FGF23 plasma concentrations differed between the groups. In cortical bone, mRNA expression levels of sclerostin (SOST), dental matrix protein 1 (DMP1), phosphate-regulating gene with homologies to endopeptidases on the X chromosome (PHEX) and matrix extracellular phosphoglycoprotein (MEPE) were lower after PT compared to ET and PET. Expression levels of CYP27b1 and vitamin D receptor (VDR) in tibial bone were decreased after PT compared to ET. In kidney, no differences between groups were observed for mRNA expression levels of CYP27b1, 24-hydroxylase (CYP24), VDR, NaPi-IIa cotransporter (NPT2a) and NaPi-IIc cotransporter (NPT2c). Serum PTH concentrations were higher after PT compared to controls. Conclusion. After six weeks, none of the training modalities induced changes in FGF23 expression levels. However, PT might have caused changes in local phosphate regulation within bone compared to ET and PET. CYP27b1 and VDR expression in bone was reduced after PT compared to ET, suggesting high intensity peak power training in this rat model is associated with decreased vitamin D signalling in bone.

KW - 1α-hydroxylation

KW - Bone

KW - Exercise

KW - FGF23

KW - Mechanical loading

KW - Phosphate homeostasis

KW - Rat

KW - Vitamin D

UR - http://www.scopus.com/inward/record.url?scp=85060644769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060644769&partnerID=8YFLogxK

U2 - 10.7717/peerj.6184

DO - 10.7717/peerj.6184

M3 - Article

VL - 2019

JO - PeerJ

JF - PeerJ

SN - 2167-8359

IS - 1

M1 - e6184

ER -

Buskermolen J, Van Der Meijden K, Furrer R, Mons DJ, Van Essen HW, Heijboer AC et al. Effects of different training modalities on phosphate homeostasis and local Vitamin D metabolism in rat bone. PeerJ. 2019 Jan 1;2019(1). e6184. https://doi.org/10.7717/peerj.6184