Effects of electron transport inhibitors and uncouplers on denitrification in Paracoccus denitrificans

F. C. Boogerd; K. J. Appeldoorn; A.H. Stouthamer

doi:10.1111/j.1574-6968.1983.tb00167.x

Effects of electron transport inhibitors and uncouplers on denitrification in Paracoccus denitrificans

F. C. Boogerd^*, K. J. Appeldoorn, A.H. Stouthamer

^*Corresponding author for this work

AIMMS

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Gaschromatographic analysis shows that whole cells of Paracoccus denitrificans produce dinitrogen in the absence and nitrous oxide in the presence of thiocyanate during nitrate reduction. NADH nitrate reductase activity in vesicles is much more sensitive to thiocyanate than either NADH oxidase activity in vesicles or reduction of nitrate by endogenous substrates in whole cells. NADH nitrate reductase activity is not inhibited and NADH oxidase activity is partially inhibited by antimycin A in vesicles. Production of nitrous oxide from nitrate in cells is completely inhibited by the simultaneous presence of thiocyanate and Triton X-100. Carbonylcyanide m-chlorophenylhydrazone does not cause a lag phase in reduction of nitrate by NADH in vesicles, in contrast to the situation in cells.

Original language	English
Pages (from-to)	455-460
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	20
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1983.tb00167.x
Publication status	Published - 1983

Keywords

Denitrification
electron transport inhibitor
membrane vesicles
nitrate uptake
Paracoccus denitrificans
uncoupler

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1574-6968.1983.tb00167.x

Persistent URL (handle)

Persistent link

Cite this

@article{ea284be93e204d78b2c1eb4d4e2feafe,

title = "Effects of electron transport inhibitors and uncouplers on denitrification in Paracoccus denitrificans",

abstract = "Gaschromatographic analysis shows that whole cells of Paracoccus denitrificans produce dinitrogen in the absence and nitrous oxide in the presence of thiocyanate during nitrate reduction. NADH nitrate reductase activity in vesicles is much more sensitive to thiocyanate than either NADH oxidase activity in vesicles or reduction of nitrate by endogenous substrates in whole cells. NADH nitrate reductase activity is not inhibited and NADH oxidase activity is partially inhibited by antimycin A in vesicles. Production of nitrous oxide from nitrate in cells is completely inhibited by the simultaneous presence of thiocyanate and Triton X-100. Carbonylcyanide m-chlorophenylhydrazone does not cause a lag phase in reduction of nitrate by NADH in vesicles, in contrast to the situation in cells.",

keywords = "Denitrification, electron transport inhibitor, membrane vesicles, nitrate uptake, Paracoccus denitrificans, uncoupler",

author = "Boogerd, {F. C.} and Appeldoorn, {K. J.} and A.H. Stouthamer",

year = "1983",

doi = "10.1111/j.1574-6968.1983.tb00167.x",

language = "English",

volume = "20",

pages = "455--460",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Effects of electron transport inhibitors and uncouplers on denitrification in Paracoccus denitrificans

AU - Boogerd, F. C.

AU - Appeldoorn, K. J.

AU - Stouthamer, A.H.

PY - 1983

Y1 - 1983

N2 - Gaschromatographic analysis shows that whole cells of Paracoccus denitrificans produce dinitrogen in the absence and nitrous oxide in the presence of thiocyanate during nitrate reduction. NADH nitrate reductase activity in vesicles is much more sensitive to thiocyanate than either NADH oxidase activity in vesicles or reduction of nitrate by endogenous substrates in whole cells. NADH nitrate reductase activity is not inhibited and NADH oxidase activity is partially inhibited by antimycin A in vesicles. Production of nitrous oxide from nitrate in cells is completely inhibited by the simultaneous presence of thiocyanate and Triton X-100. Carbonylcyanide m-chlorophenylhydrazone does not cause a lag phase in reduction of nitrate by NADH in vesicles, in contrast to the situation in cells.

AB - Gaschromatographic analysis shows that whole cells of Paracoccus denitrificans produce dinitrogen in the absence and nitrous oxide in the presence of thiocyanate during nitrate reduction. NADH nitrate reductase activity in vesicles is much more sensitive to thiocyanate than either NADH oxidase activity in vesicles or reduction of nitrate by endogenous substrates in whole cells. NADH nitrate reductase activity is not inhibited and NADH oxidase activity is partially inhibited by antimycin A in vesicles. Production of nitrous oxide from nitrate in cells is completely inhibited by the simultaneous presence of thiocyanate and Triton X-100. Carbonylcyanide m-chlorophenylhydrazone does not cause a lag phase in reduction of nitrate by NADH in vesicles, in contrast to the situation in cells.

KW - Denitrification

KW - electron transport inhibitor

KW - membrane vesicles

KW - nitrate uptake

KW - Paracoccus denitrificans

KW - uncoupler

UR - http://www.scopus.com/inward/record.url?scp=0021043219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021043219&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.1983.tb00167.x

DO - 10.1111/j.1574-6968.1983.tb00167.x

M3 - Article

AN - SCOPUS:0021043219

SN - 0378-1097

VL - 20

SP - 455

EP - 460

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

Effects of electron transport inhibitors and uncouplers on denitrification in Paracoccus denitrificans

Abstract

Keywords

UN SDGs

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this